This study reports the effect of cigarette smoking on serum and salivary -amylase activities of some consenting Nigerian male smokers (20) in apparent good health and between 30 to 70 years. Age and sex-matched non-smokers (20) were included as control subjects. Alpha amylase activity was assayed using standard colorimetric method. Results obtained show that the mean α-amylase activity in serum and saliva were significantly higher (P<0.05) in smokers (131.2 ± 17.0 IU/L and 241.8 ± 23.6 IU/L) when compared with non-smokers (68.3 ± 11.3 IU/L and 133.5 ± 8.6 IU/L), and these represent 84.3% and 95.2% increases in serum and saliva values for the smokers relative to the non-smokers. Evidence from this study indicates a measure of pancreatic, salivary and parotid glands’ dysfunction among the cigarette smokers in Nigeria. Measurement of serum and salivary - amylase activity could be useful in the early detection and diagnosis of pancreatitis and parotitis.