An efficient and reproducible procedure was established for direct shoot regeneration of Solanum surattense Bum. using internodal explants. The shoot regeneration efficiency on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) was superior in compared to 6-furfurylaminopurine (Kn). The highest mean number of shoots per explant was recorded on MS medium containing 0.5 mgl-1 BAP. BAP alone was more effective for shoot regeneration than combination of BAP and α-naphthaleneacetic acid (NAA). The number of shoot per explant was highest in the 4th subculture and then it was remained stable up to 6th subculture. The shoot number drastically reduced from 7th subculture when explants were repeated subculturing up to 8th subculture. Hundred percent adventitious shoots were rooted successively on half strength MS medium fortified with 0.05 mgl-1 NAA. At about 90% of the in vitro-propagated plantlets survived when they were transferred to ex vitro conditions for acclimatization.