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PLOS ONE  2012 

Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2

DOI: 10.1371/journal.pone.0044027

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Abstract:

Low-power laser irradiation (LPLI) has been found to induce various biological effects and cellular processes. Also, LPLI has been shown to promote fracture repair. Until now, it has been unclear how LPLI promotes bone formation and fracture healing. The aim of this study was to investigate the potential mechanism of LPLI-mediated enhancement of bone formation using mouse bone marrow mesenchymal stem cells (D1 cells). D1 cells were irradiated daily with a gallium-aluminum-arsenide (GaAlAs) laser at dose of 0, 1, 2, or 4 J/cm2. The lactate dehydrogenase (LDH) assay showed no cytotoxic effects of LPLI on D1 cells, and instead, LPLI at 4 J/cm2 significantly promoted D1 cell proliferation. LPLI also enhanced osteogenic differentiation in a dose-dependent manner and moderately increased expression of osteogenic markers. The neutralization experiments indicated that LPLI regulated insulin-like growth factor 1 (IGF1) and bone morphogenetic protein 2 (BMP2) signaling to promote cell proliferation and/or osteogenic differentiation. In conclusion, our study suggests that LPLI may induce IGF1 expression to promote both the proliferation and osteogenic differentiation of D1 cells, whereas it may induce BMP2 expression primarily to enhance osteogenic differentiation.

References

[1]  Riggs BL, Melton LJ, 3rd (1995) The worldwide problem of osteoporosis: insights afforded by epidemiology. Bone 17: 505S–511S.
[2]  Totosy de Zepetnek JO, Giangregorio LM, Craven BC (2009) Whole-body vibration as potential intervention for people with low bone mineral density and osteoporosis: a review. J Rehabil Res Dev 46: 529–542.
[3]  Pietschmann P, Rauner M, Sipos W, Kerschan-Schindl K (2009) Osteoporosis: an age-related and gender-specific disease–a mini-review. Gerontology 55: 3–12.
[4]  Mehler PS, MacKenzie TD (2009) Treatment of osteopenia and osteoporosis in anorexia nervosa: a systematic review of the literature. Int J Eat Disord 42: 195–201.
[5]  Dahir GA, Cui Q, Anderson P, Simon C, Joyner C, et al.. (2000) Pluripotential mesenchymal cells repopulate bone marrow and retain osteogenic properties. Clin Orthop Relat Res: S134–145.
[6]  Chen CH, Ho ML, Chang JK, Hung SH, Wang GJ (2005) Green tea catechin enhances osteogenesis in a bone marrow mesenchymal stem cell line. Osteoporos Int 16: 2039–2045.
[7]  Arany PR, Nayak RS, Hallikerimath S, Limaye AM, Kale AD, et al. (2007) Activation of latent TGF-beta1 by low-power laser in vitro correlates with increased TGF-beta1 levels in laser-enhanced oral wound healing. Wound Repair Regen 15: 866–874.
[8]  Gal P, Mokry M, Vidinsky B, Kilik R, Depta F, et al. (2009) Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and corticosteroid-treated rats. Lasers Med Sci 24: 539–547.
[9]  Iijima K, Shimoyama N, Shimoyama M, Yamamoto T, Shimizu T, et al. (1989) Effect of repeated irradiation of low-power He-Ne laser in pain relief from postherpetic neuralgia. Clin J Pain 5: 271–274.
[10]  Sakurai Y, Yamaguchi M, Abiko Y (2000) Inhibitory effect of low-level laser irradiation on LPS-stimulated prostaglandin E2 production and cyclooxygenase-2 in human gingival fibroblasts. Eur J Oral Sci 108: 29–34.
[11]  Schindl A, Merwald H, Schindl L, Kaun C, Wojta J (2003) Direct stimulatory effect of low-intensity 670 nm laser irradiation on human endothelial cell proliferation. Br J Dermatol 148: 334–336.
[12]  Taniguchi D, Dai P, Hojo T, Yamaoka Y, Kubo T, et al. (2009) Low-energy laser irradiation promotes synovial fibroblast proliferation by modulating p15 subcellular localization. Lasers Surg Med 41: 232–239.
[13]  Tuby H, Maltz L, Oron U (2007) Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg Med 39: 373–378.
[14]  Hou JF, Zhang H, Yuan X, Li J, Wei YJ, et al. (2008) In vitro effects of low-level laser irradiation for bone marrow mesenchymal stem cells: proliferation, growth factors secretion and myogenic differentiation. Lasers Surg Med 40: 726–733.
[15]  Barushka O, Yaakobi T, Oron U (1995) Effect of low-energy laser (He-Ne) irradiation on the process of bone repair in the rat tibia. Bone 16: 47–55.
[16]  Ninomiya T, Miyamoto Y, Ito T, Yamashita A, Wakita M, et al. (2003) High-intensity pulsed laser irradiation accelerates bone formation in metaphyseal trabecular bone in rat femur. J Bone Miner Metab 21: 67–73.
[17]  Pretel H, Lizarelli RF, Ramalho LT (2007) Effect of low-level laser therapy on bone repair: histological study in rats. Lasers Surg Med 39: 788–796.
[18]  Arisu HD, Turkoz E, Bala O (2006) Effects of Nd : Yag laser irradiation on osteoblast cell cultures. Lasers in Medical Science 21: 175–180.
[19]  Soares CP, Oliveira DAAP, de Oliveira RF, Zangaro RA (2008) Evaluation of low-level laser therapy of osteoblastic cells. Photomedicine and Laser Surgery 26: 401–404.
[20]  Stein E, Koehn J, Sutter W, Wendtlandt G, Wanschitz F, et al. (2008) Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wien Klin Wochenschr 120: 112–117.
[21]  Diduch DR, Coe MR, Joyner C, Owen ME, Balian G (1993) Two cell lines from bone marrow that differ in terms of collagen synthesis, osteogenic characteristics, and matrix mineralization. J Bone Joint Surg Am 75: 92–105.
[22]  Wang YH, Ho ML, Chang JK, Chu HC, Lai SC, et al. (2009) Microporation is a valuable transfection method for gene expression in human adipose tissue-derived stem cells. Mol Ther 17: 302–308.
[23]  Moore NM, Lin NJ, Gallant ND, Becker ML (2011) Synergistic enhancement of human bone marrow stromal cell proliferation and osteogenic differentiation on BMP-2-derived and RGD peptide concentration gradients. Acta Biomater 7: 2091–2100.
[24]  Cen S, Zhang J, Huang F, Yang Z, Xie H (2008) [Effect of IGF-1 on proliferation and differentiation of primary human embryonic myoblasts]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 22: 84–87.
[25]  Jin DM, Chen LL, Yan J (2006) [Effects of IGF-I and BMP-2 combined application on promoting proliferation, differentiation and calcification of MC 3T3-E1 and NIH 3T3 cells]. Zhejiang Da Xue Xue Bao Yi Xue Ban 35: 55–63.
[26]  Wildemann B, Schmidmaier G, Ordel S, Stange R, Haas NP, et al. (2003) Cell proliferation and differentiation during fracture healing are influenced by locally applied IGF-I and TGF-beta1: comparison of two proliferation markers, PCNA and BrdU. J Biomed Mater Res B Appl Biomater 65: 150–156.
[27]  Huang X, Lee C (2003) Regulation of stromal proliferation, growth arrest, differentiation and apoptosis in benign prostatic hyperplasia by TGF-beta. Front Biosci 8: s740–749.
[28]  Baron W, Metz B, Bansal R, Hoekstra D, de Vries H (2000) PDGF and FGF-2 signaling in oligodendrocyte progenitor cells: regulation of proliferation and differentiation by multiple intracellular signaling pathways. Mol Cell Neurosci 15: 314–329.
[29]  Coombe AR, Ho CT, Darendeliler MA, Hunter N, Philips JR, et al. (2001) The effects of low level laser irradiation on osteoblastic cells. Clin Orthod Res 4: 3–14.
[30]  Mvula B, Mathope T, Moore T, Abrahamse H (2008) The effect of low level laser irradiation on adult human adipose derived stem cells. Lasers Med Sci 23: 277–282.
[31]  Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22: 347–354.
[32]  Eduardo Fde P, Bueno DF, de Freitas PM, Marques MM, Passos-Bueno MR, et al. (2008) Stem cell proliferation under low intensity laser irradiation: a preliminary study. Lasers Surg Med 40: 433–438.
[33]  Horvat-Karajz K, Balogh Z, Kovacs V, Drrernat AH, Sreter L, et al. (2009) In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells. Lasers Surg Med 41: 463–469.
[34]  Xu M, Deng T, Mo F, Deng B, Lam W, et al. (2009) Low-intensity pulsed laser irradiation affects RANKL and OPG mRNA expression in rat calvarial cells. Photomedicine and Laser Surgery 27: 309–315.
[35]  Fukuhara E, Goto T, Matayoshi T, Kobayashi S, Takahashi T (2006) Optimal low-energy laser irradiation causes temporal G2/M arrest on rat calvarial osteoblasts. Calcif Tissue Int 79: 443–450.
[36]  Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 26: 3503–3509.
[37]  Bouvet-Gerbettaz S, Merigo E, Rocca JP, Carle GF, Rochet N (2009) Effects of low-level laser therapy on proliferation and differentiation of murine bone marrow cells into osteoblasts and osteoclasts. Lasers Surg Med 41: 291–297.
[38]  Hamajima S, Hiratsuka K, Kiyama-Kishikawa M, Tagawa T, Kawahara M, et al. (2003) Effect of low-level laser irradiation on osteoglycin gene expression in osteoblasts. Lasers Med Sci 18: 78–82.
[39]  Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomedicine and Laser Surgery 23: 161–166.
[40]  Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, et al. (1999) Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev 20: 345–357.
[41]  Khadra M, Ronold HJ, Lyngstadaas SP, Ellingsen JE, Haanaes HR (2004) Low-level laser therapy stimulates bone-implant interaction: an experimental study in rabbits. Clin Oral Implants Res 15: 325–332.

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