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PLOS ONE  2012 

Isolation and Evaluation of Oil-Producing Microalgae from Subtropical Coastal and Brackish Waters

DOI: 10.1371/journal.pone.0040751

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Abstract:

Microalgae have been widely reported as a promising source of biofuels, mainly based on their high areal productivity of biomass and lipids as triacylglycerides and the possibility for cultivation on non-arable land. The isolation and selection of suitable strains that are robust and display high growth and lipid accumulation rates is an important prerequisite for their successful cultivation as a bioenergy source, a process that can be compared to the initial selection and domestication of agricultural crops. We developed standard protocols for the isolation and cultivation for a range of marine and brackish microalgae. By comparing growth rates and lipid productivity, we assessed the potential of subtropical coastal and brackish microalgae for the production of biodiesel and other oil-based bioproducts. This study identified Nannochloropsis sp., Dunaniella salina and new isolates of Chlorella sp. and Tetraselmis sp. as suitable candidates for a multiple-product algae crop. We conclude that subtropical coastal microalgae display a variety of fatty acid profiles that offer a wide scope for several oil-based bioproducts, including biodiesel and omega-3 fatty acids. A biorefinery approach for microalgae would make economical production more feasible but challenges remain for efficient harvesting and extraction processes for some species.

References

[1]  Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25: 294–306.
[2]  Malcata FX (2011) Microalgae and biofuels: A promising partnership? Trends Biotechnol 29: 542–549.
[3]  Schenk PM, Thomas-Hall SR, Stephens E, Marx UC, Mussgnug JH, et al. (2008) Second generation biofuels: high-efficiency microalgae for biodiesel production. Bioenerg Res 1: 20–43.
[4]  Dermirbas A (2009) Potential resources of non-edible oils for biodiesel. Energy Sources Part B – Economics Planning & Policy 4: 310–314.
[5]  Durret T, Benning C, Ohlrogge J (2008) Plant triacylglycerols as feedstocks for the production of biofuels. Plant J 54: 593–607.
[6]  Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, et al. (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54: 621–639.
[7]  Ahmad AL, Mat Yasin NH, Derek CJC, Lim JK (2011) Microalgae as a sustainable energy source for biodiesel production: A review. Renew Sustain Energ Rev 15: 584–593.
[8]  Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26: 126–131.
[9]  Gouveia L, Oliveria A (2009) Microalgae as a raw material for biofuels production. J Ind Microbiol Biotechnol 36: 269–274.
[10]  Kliphus AMJ, Lenneke DW, Vejrazka C (2010) Photosynthetic Efficiency of Chlorella sorokiana in a turbulently mixed short light-path photobioreactor. Biotechnol Progr 26: 687–696.
[11]  Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, et al. (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102: 100–112.
[12]  Zeiler KG, Heacox DA, Toon ST, Kadam KL, Brown LM (1995) The use of microalgae for assimilation and utlization of carbon dioxide from fossil fuel-fired power plant flue gas. Energ Convers Manag 36: 702–712.
[13]  Wang B, Li YQ, Wu N, Lan CQ (2008) CO2 bio-mitigation using microalgae. Appl Microbiol Biotechnol 79: 707–718.
[14]  Bridgewater A, Maniatis K (2004) The production of biofuels by thermal chemical processing of biomass. In: Archer M, Barber J, editors. London: Imperial College Press.
[15]  Pulz O, Gross W (2004) Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 65: 635–648.
[16]  Ruxton CHS, Reed SC, Simpson MJA, Millington KJ (2004) The health benefits of omega-3 polyunsaturated fatty acids: a review of the evidence. J Human Nutrition Dietetics 17: 449–459.
[17]  Cavalli RO, Lavens P, Sorgeloos P (1999) Performance of Macrobrachium rosenbergii broodstock fed diets with different fatty acid composition. Aquaculture 179: 387–402.
[18]  Doroudi MS, Southgate PC, Mayer RJ (1999) Growth and survival of blacklip pearl oyster larvae fed different densities of microalgae. Aquaculture Internat 7: 179–187.
[19]  Emata AC, Ogata HY, Garibay ES, Furuita H (2003) Advanced broodstock diets for the mangrove red snapper and a potential importance of arachidonic acid in eggs and fry. Fish Physiol Biochem 28: 489–491.
[20]  Amaro HM, Guedes AC, Malcata FX (2011) Advances and perspectives in using microalgae to produce biodiesel. Appl Energy 88: 3402–3410.
[21]  Weyer KM, Bush DR, Darzins A (2010) Theoretical maximum algal oil production. Bioenerg Res 3: 204–213.
[22]  Dermirbas A, Dermirbas MF (2011) Importance of algae oil as a source of biodiesel. Energy Convers Manag 52: 163–170.
[23]  Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: A review. Renew Sustain Energ Rev 14: 217–232.
[24]  Knothe G, Gerpen JV, Krahl J (2005) The biodiesel handbook. Urbana, IL: AOCS Press.
[25]  Knothe G (2005) Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters. Fuel Process Technol 86: 1059–1070.
[26]  Abu-Rezq T, Al-Musallam L, Al-Shimmari J (1999) Optimum production conditions for different high-quality marine algae. Hydrobiologia 403: 91–107.
[27]  Chiu SY, Kao CY, Tsai MT, Ong SC, Chen CH, et al. (2009) Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. Bioresour Technol 100: 833–838.
[28]  Cho S, Ji SC, Hur S, Bae J, Park IS, et al. (2007) Optimum temperature and salinity conditions for growth of green algae Chlorella ellipsoidea and Nannochloris oculata. Fish Science 73: 1050–1056.
[29]  Li YQ, Horsman M, Wang B, Wu N, Lan CQ (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green algae Neochloris oleoabundans. Appl Microbiol Biotechnol 81: 629–636.
[30]  Chen M, Tang H, Ma H, Holland TC, Ng KYS, et al. (2011) Effects of nutrient on growth and lipid accumulation in the green algae Dunaliella tertiolecta. Bioresour Technol 102: 1649–1655.
[31]  Huerlimann R, de Nys R, Heimann K (2010) Growth, lipid content, productivity, and fatty acid composition of tropical microalgae for scale-up production. Biotechnol Bioeng 107: 245–257.
[32]  Renaud SM, Thinh LV, Lambrinidis G, Parry DL (2002) Effect of temperature on growth, chemical composition and fatty acid composition of tropical Australian microalgae grown in batch cultures. Aquaculture 211: 195–214.
[33]  Araujo GS, Matos LJBL, Goncalves LRB, Fernandes FAN, Farias WRL (2011) Bioprospecting for oil producing microalgal strains: Evaluation of oil and biomass production for ten microalgal strains. Bioresour Technol 102: 5248–5250.
[34]  Lee S, Go S, Jeong G, Kim S (2011) Oil production from five marine microalgae for the production of biodiesel. Biotechnol Bioprocess Eng 16: 561–566.
[35]  Patil V, Kallqvist T, Olsen E, Vogt G, Gislerod HR (2007) Fatty acid composition of 12 microalgae for possible use in aquaculture feed. Aquaculture Internat 15: 1–9.
[36]  Converti A, Casazza AA, Ortiz EY, Perego P, Del Borghi M (2009) Effects of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris. Chem Eng Process 48: 1146–1151.
[37]  Illman AM, Scragg AH, Shales SW (2000) Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzyme Microb Technol 27: 631–635.
[38]  Borowitzka MA (1988) Fats, oils and hydrocarbons. Borowitzka, MA and LJ Borowitzka (Ed) Micro-Algal Biotechnology X+477p Cambridge University Press: New York, New York, USA; Cambridge, England, UK Illus. pp. 257–287.
[39]  Roessler PG (1990) Environmental control of glycerolipid metabolism in microalgae – commercial implications and future-research directions. J Phycol 26: 393–399.
[40]  de la Pena MR, Villegas CT (2005) Cell growth, effect of filtrate and nutritive value of the tropical prasinophyte Tetraselmis tetrathele (Butcher) at different phases of culture. Aquaculture Res 36: 1500–1508.
[41]  Guillard RR, Ryther JH (1962) Studies of marine planktonic diatoms: I. Cyclotella nana (Hustedt) and Detonula confervacea (Cleve) Gran. Canad J Microbiol 8: 229–239.
[42]  Lorenz M, Friedl T, Day JG (2005) Perpetual maintenance of actively metabolizing microalgal cultures. In: Andersen RA, editor. pp. 145–156. Burlington, MA: Elsevier Academic Press.
[43]  Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate phenol chloroform extraction. Analyt Biochem 162: 156–159.
[44]  Katoh K, Asimenos G, Toh H (2009) Multiple alignment of DNA sequences with MAFFT. Meth Mol Biol 537: 39–64.
[45]  Guidon S, Gascuel O (2003) A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Systems Biol 52: 696–704.
[46]  Wood AM, Everroad RC, Wingard LM (2005) Chapter 18: Measuring growth rates in microalgal cluures. In: A AR, editor. pp. 269–285. Burlington, MA: Elsevier Academic Press.
[47]  Pal D, Khozin-Goldberg I, Cohin Z, Boussiba S (2011) The effect of light, salinity and nitrogen availability on lipid production by Nannochloropsis sp. Appl Microbiol Biotechnol 90: 1429–1441.
[48]  Araujo SC, Garcia VMT (2005) Growth and biochemical composition of the diatom Chaetoceros cf. wighamii Brightwell under different temperature, salinity and carbon dioxide levels. I. Protein, carbohydrates and lipids. Aquaculture 246: 405–412.
[49]  Emdadi D, Berland B (1989) Variation in lipid class composition during batch growth of Nannochloropsis salina and Pavlova lutheri. Marine Chem 26: 215–225.
[50]  Rocha JMS, Garcia JEC, Henriques MHF (2003) Growth aspects of the marine microalga Nannochloropsis gaditana. Biomol Eng 20: 237–242.
[51]  Miyamoto K, Wable O, Benemann JR (1988) Vertical tubular reactor for microalgae cultivation. Biotechnol Lett 10: 703–708.
[52]  Alonzo F, Mayzaud P (1999) Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile red. Marine Chem 67: 289–301.
[53]  Gong Y, Jiang M (2011) Biodiesel production with microalgae as feedstock: from strains to biodiesel. Biotechnol Lett 33: 1269–1284.
[54]  Hu H, Gao K (2003) Optimisation of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enrichment carbon sources. Biotechnol Lett 25: 421–425.
[55]  Chen CH, Yeh K, Su H, Lo Y, Chen W, et al. (2010) Strategies to enhance cell growth and achieve high-level oil production of a Chlorella vulgaris isolate. Biotechnol Prog 26: 679–686.
[56]  Knothe G (2008) “Designer” biodiesel: optimising fatty ester composition to improve fuel properties. Energy Fuels 22: 1358–1364.
[57]  Reitan KI, Rainuzzo JR, Olsen Y (1994) Effect of nutrient limitation on fatty acid and lipid content of marine microalgae. J Phycol 30: 972–979.
[58]  Jeh EJ, Song SK, Seo JW, Hur BK (2007) Variation in the lipid class and fatty acid composition of Thraustochytrium aureum ATCC 34304. Korean J Biotechnol Bioeng 22: 37–42.
[59]  Dunstan GH, Volkman JK, Barret SM, Garland CD (1993) Changes in the lipid composition and maximization of the polyunsaturated fatty acid content of three microalgae grown in mass culture. J Appl Phycol 7: 71–83.
[60]  Shamsudin L (1992) Lipid and fatty acid composition of microalgae used in Malaysian aquaculture as live food for the early stage of penaeid larvae. J Appl Phycol 4: 371–378.
[61]  Okauchi M, Kawamura K (1997) Optimum medium for large-scale culture of Tetraselmis tetrathele. Hydrobiologia 358: 217–222.

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