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PLOS ONE  2012 

Lichtheimia Species Exhibit Differences in Virulence Potential

DOI: 10.1371/journal.pone.0040908

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Abstract:

Although the number of mucormycosis cases has increased during the last decades, little is known about the pathogenic potential of most mucoralean fungi. Lichtheimia species represent the second and third most common cause of mucormycosis in Europe and worldwide, respectively. To date only three of the five species of the genus have been found to be involved in mucormycosis, namely L. corymbifera, L. ramosa and L. ornata. However, it is not clear whether the clinical situation reflects differences in virulence between the species of Lichtheimia or whether other factors are responsible. In this study the virulence of 46 strains of all five species of Lichtheimia was investigated in chicken embryos. Additionally, strains of the closest-related genus Dichotomocladium were tested. Full virulence was restricted to the clinically relevant species while all strains of L. hyalospora, L. sphaerocystis and Dichotomocladium species were attenuated. Although virulence differences were present in the clinically relevant species, no connection between origin (environmental vs clinical) or phylogenetic position within the species was observed. Physiological studies revealed no clear connection of stress resistance and carbon source utilization with the virulence of the strains. Slower growth at 37°C might explain low virulence of L. hyalospora, L. spaherocystis and Dichotomocladium; however, similarly slow growing strains of L. ornata were fully virulent. Thus, additional factors or a complex interplay of factors determines the virulence of strains. Our data suggest that the clinical situation in fact reflects different virulence potentials in the Lichtheimiaceae.

References

[1]  Roden MM, Zaoutis TE, Buchanan WL, Knudsen TA, Sarkisova TA, et al. (2005) Epidemiology and outcome of zygomycosis: A review of 929 reported cases. Clin Infect Dis 41: 634–653.
[2]  Fairley C, Sullivan TJ, Bartley P, Allworth T, Lewandowski R (2000) Survival after rhino-orbital-cerebral mucormycosis in an immunocompetent patient. Ophthalmology 107: 555–558.
[3]  Gomes MZ, Lewis RE, Kontoyiannis DP (2011) Mucormycosis caused by unusual mucormycetes, non-Rhizopus, -Mucor, and -Lichtheimia species. Clin Microbiol Rev 24: 411–445.
[4]  Hussain S, Salahuddin N, Ahmad I, Salahuddin I, Jooma R (1995) Rhinocerebral invasive mycosis: occurrence in immunocompetent individuals. Eur J Radiol 20: 151–155.
[5]  Liang KP, Tleyjeh IM, Wilson WR, Roberts GD, Temesgen Z (2006) Rhino-orbitocerebral mucormycosis caused by Apophysomyces elegans. J Clin Microbiol 44: 892–898.
[6]  Tsung LL, Zhu XL, Chu WC, Sun DT, Cheung KL, et al. (2010) Intraventricular amphotericin for absidiomycosis in an immunocompetent child. Hong Kong Med J 16: 137–140.
[7]  Lanternier F, Dannaoui E, Morizot G, Elie C, Garcia-Hermoso D, et al. (2012) A Global Analysis of Mucormycosis in France: The RetroZygo Study (2005–2007). Clin Infect Dis 54: S35–43.
[8]  Skiada A, Pagano L, Groll A, Zimmerli S, Dupont B, et al. (2011) Zygomycosis in Europe: analysis of 230 cases accrued by the registry of the European Confederation of Medical Mycology (ECMM) Working Group on Zygomycosis between 2005 and 2007. Clin Microbiol Infect 17: 1859–1867.
[9]  Alastruey-Izquierdo A, Hoffmann K, de Hoog GS, Rodriguez-Tudela JL, Voigt K, et al. (2010) Species recognition and clinical relevance of the zygomycetous genus Lichtheimia (syn. Absidia Pro Parte, Mycocladus). J Clin Microbiol 48: 2154–2170.
[10]  Bates S, Hughes HB, Munro CA, Thomas WPH, MacCallum DM, et al. (2005) Outer Chain N-Glycans Are Required for Cell Wall Integrity and Virulence of Candida albicans. The Journal of Biological Chemistry 281: 90–98.
[11]  Duran R, Cary JW, Calvo AM (2010) Role of the Osmotic Stress Regulatory Pathway in Morphogenesis and Secondary Metabolism in Filamentous Fungi. Toxins 2: 367–381.
[12]  Nakagawa Y, Kanbe T, Mizuguchi I (2003) Disruption of the human pathogenic yeast Candida albicans catalase gene decreases survival in mouse-model infection and elevates susceptibility to higher temperature and to detergents. Microbiol Immunol 47: 395–403.
[13]  Brock M (2009) Fungal metabolism in host niches. Current Opinion in Microbiology 12: 371–376.
[14]  Cooney NM, Klein BS (2008) Fungal adaptation to the mammalian host: it is a new world, after all. Current Opinion in Microbiology 11: 511–516.
[15]  Champion OL, Cooper IAM, James SL, Ford D, Karlyshev A, et al. (2009) Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis. Microbiology 155: 1516–1522.
[16]  Lamaris GA, Ben-Ami R, Lewis RE, Chamilos G, Samonis G, et al. (2009) Increased Virulence of Zygomycetes Organisms Following Exposure to Voriconazole: A Study Involving Fly and Murine Models of Zygomycosis. The Journal of infectious diseases 199: 1399–1406.
[17]  Cymborowski B (2000) Temperature-dependent regulatory mechanism of larval development of the wax moth (Galleria mellonella). Acta Biochemica Polonica 47 (1): 215–221.
[18]  Ewbank JJ, Zugasti O (2011) C. elegans: model host and tool for antimicrobial drug discovery. Disease Models & Mechanisms 4: 1–5.
[19]  Kavanagh K, Reeves EP (2007) Insect and Mammalian Innate Immune Responses Are Much Alike. Microbe 2 (12): 596–599.
[20]  H?rtl A, Hillesheim HG, Kunkel W, Schrinner EJ (1995) The Candida infected hen’s egg. An alternative test system for systemic anticandida activity. Arzneimittelforschung 45: 926–928.
[21]  Tieffenberg J, Vogel L, Kretschmer RR, Padnos D, Gotoff SP (1978) Chicken Embryo Model for Type III Group B Beta-Hemolytic Streptococcal Septicemia. Infect Immun 19: 481–485.
[22]  Jacobsen ID, Gro?e K, Slesiona S, Hube B, Berndt A, et al. (2010) Embryonated eggs as an alternative infection model to investigate Aspergillus fumigatus virulence. Infect Immun 78: 2995–3006.
[23]  Garcia-Hermoso D, Hoinard D, Gantier JC, Grenouillet F, Dromer F, et al. (2009) Molecular and Phenotypic Evaluation of Lichtheimia corymbifera (Formerly Absidia corymbifera) Complex Isolates Associated with Human Mucormycosis: Rehabilitation of L. ramosa. J Clin Microbiol 47: 3862–3870.
[24]  Jacobsen ID, Gro?e K, Berndt A, Hube B (2011) Pathogenesis of Candida albicans Infections in the Alternative Chorio-Allantoic Membrane Chicken Embryo Model Resembles Systemic Murine Infections. Plos One 6: e19741.
[25]  Ribes JA, Vanover-Sams CL, Baker DJ (2000) Zygomycetes in Human Disease. Clinical Microbiology Reviews 13: 236–301.
[26]  Sugar AM (1992) Mucormycosis. Clin Infect Dis 14: 126–129.
[27]  Ibrahim A, Spellberg B, Avanessian V, Fu Y, Edwards Jr JE (2005) Rhizopus oryzae Adheres to, Is Phagocytosed by, and Damages Endothelial Cells In Vitro. Infect Immun 73: 778–783.
[28]  Liu M, Spellberg B, Phan QT, Fu Y, Fu Y, et al. (2010) The endothelial cell receptor GRP78 is required for mucormycosis pathogenesis in diabetic mice. J Clin Invest 120: 1914–1924.
[29]  Aufauvre-Brown A, Brown JS, Holden DW (1998) Comparison of virulence between clinical and environmental isolates of Aspergillus fumigatus. Eur J Clin Microbiol Infect Dis 17: 778–780.
[30]  Delgado AC, Taguchi H, Mikami Y, Myiajy M, Villares MC, et al. (2005) Human cryptococcosis: relationship of environmental and clinical strains of Cryptococcus neoformans var. neoformans from urban and rural areas. Mycopathologia 159: 7–11.
[31]  Fromtling RA, Abruzzo GK, Ruiz A (1989) Virulence and antifungal susceptibility of environmental and clinical isolates of Cryptococcus neoformans from Puerto Rico. Mycopathologia 106: 163–166.
[32]  Olias P, Gruber AD, Hafez HM, Lierz M, Slesiona S, et al. (2011) Molecular epidemiology and virulence assessment of Aspergillus fumigatus isolates from white stork chicks and their environment. Vet Microbiol 148: 348–355.
[33]  McCormick A, Loeffler J, Ebel F (2010) Aspergillus fumigatus: contours of an opportunistic human pathogen. Cellular Microbiology 12: 1535–1543.
[34]  Detilleux PG, Cheville NF, Deyoe BL (1988) Pathogenesis of Brucella abortus in Chicken Embryos. Vet Pathol 25: 138–146.
[35]  W?stemeyer J (1985) Strain-dependent variation in ribosomal DNA arrangement in Absidia glauca. Eur J Biochem 146: 443–448.
[36]  Cenis JL (1993) Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res 20: 2380.
[37]  Hoffmann K, Discher S, Voigt K (2007) Revision of the genus Absidia (Mucorales, Zygomycetes) based on physiological, phylogenetic, and morphological characters; thermotolerant Absidia spp. form a coherent group, Mycocladiaceae fam. nov. Mycol Res 111: 1169–1183.
[38]  White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, In: Innis, Gelfand, Sninsky, White, PCR Protocols: A Guide to Methods and Applications p. 315 p.
[39]  O’Donnell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor J W, editors. The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. pp. 225–233. editors.
[40]  O’Donnell K, Cigelnik E, Benny GL (1998) Phylogenetic Relationships among the Harpellales and Kickxellales. Mycologia 90: 624–639.
[41]  Vogelstein B, Gillspie D (1979) Preparative and analytical purification of DNA from agarose. PNAS 76: 615–619.
[42]  Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680.
[43]  Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41: 95–98.
[44]  Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30: 3059–3066.
[45]  Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425.
[46]  Michener CD, Sokal RR (1957) A quantitative approach to a problem of classification. Evolution 11: 490–499.
[47]  Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.

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