All Title Author
Keywords Abstract

PLOS ONE  2011 

Variation in the Male Pheromones and Mating Success of Wild Caught Drosophila melanogaster

DOI: 10.1371/journal.pone.0023645

Full-Text   Cite this paper   Add to My Lib

Abstract:

Drosophila melanogaster males express two primary cuticular hydrocarbons (male-predominant hydrocarbons). These act as sex pheromones by influencing female receptivity to mating. The relative quantities of these hydrocarbons vary widely among natural populations and can contribute to variation in mating success. We tested four isofemale lines collected from a wild population to assess the effect of intrapopulation variation in male-predominant hydrocarbons on mating success. The receptivity of laboratory females to males of the four wild-caught lines varied significantly, but not consistently in the direction predicted by variation in male-predominant hydrocarbons. Receptivity of the wild-caught females to laboratory males also varied significantly, but females from lines with male-predominant hydrocarbon profiles closer to a more cosmopolitan one did not show a correspondingly strong mating bias toward a cosmopolitan male. Among wild-caught lines, the male-specific ejaculatory bulb lipid, cis-vaccenyl acetate, varied more than two-fold, but was not associated with variation in male mating success. We observed a strong inverse relationship between the receptivity of wild-caught females and the mating success of males from their own lines, when tested with laboratory flies of the opposite sex.

References

[1]  Jallon J-M (1984) A few chemical words exchanged during courtship and mating of Drosophila melanogaster. Behav Genet 14: 441–478.
[2]  Scott D (1994) Genetic variation for female mate discrimination in Drosophila melanogaster. Evolution 48: 112–121.
[3]  Van Den Berg MJ, Thomas G, Hendricks M, Van Delden W (1984) A reexamination of the negative assortative mating phenomenon and its underlying mechanisms in Drosophila melanogaster. Behav Genet 14: 45–61.
[4]  Carson HL (2002) Female choice in Drosophila: evidence from Hawaii and implications for evolutionary biology. Genetica 116: 383–393.
[5]  Etges WJ, Tripodi AD (2008) Premating isolation is determined by larval rearing substrates in cactophilic Drosophila mojavensis. VIII. Mating success mediated by epicuticular hydrocarbons within and between isolated populations. J Evol Biol 21: 1641–1652.
[6]  Etges WJ, Oliveira CC, Ritchie MG, Noor MAF (2009) Genetics of incipient speciation in Drosophila mojavensis. II Host plants and mating status influence cuticular hydrocarbon QTL expression and G x E interactions. Evolution 63: 1712–1730.
[7]  Etges WJ, Oliveira CC, Noor MAF, Ritchie MG (2010) Genetics of incipient speciation in Drosophila mojavensis. III Life history divergence and reproductive isolation. Evolution 64: 3549–3569.
[8]  Antony C, Davis TL, Carlson DA, Pechine J-M, Jallon J-M (1985) Compared behavioral responses of male Drosophila melanogaster Canton-S to natural and synthetic aphrodisiacs. J of Chem Ecol 11: 1617–1629.
[9]  Ferveur J-F, Cobb M, Boukella H, Jallon J-M (1996) Worldwide variation in Drosophila melanogaster sex pheromone: behavioral effects genetic bases and potential evolutionary consequences. Genetica 97: 73–80.
[10]  Ferveur J-F, Jallon J-M (1996) Genetic control of male cuticular hydrocarbons in Drosophila melanogaster. Genet Res 67: 211–218.
[11]  Scott D, Richmond RC (1988) A genetic analysis of male-predominant pheromones in Drosophila melanogaster. Genetics 119: 639–646.
[12]  Scott D, Jackson L (1988) Interstrain comparison of male-predominant antiaphrodisiacs in Drosophila melanogaster. J Insect Physiol 34: 863–871.
[13]  Spieth HT (1974) Courtship behavior in Drosophila. Ann Rev Entomol 19: 383–406.
[14]  Tompkins L (1984) Genetic analysis of sex appeal in Drosophila. Behav Gene. 14. : 411–440.
[15]  Scott D, Richmond RC, Carlson DA (1988) Pheromones exchanged during mating: a mechanism for mate assessment in Drosophila. Anim Behav 36: 1164–1173.
[16]  Coyne JA, Wicker-Thomas C, Jallon J-M (1999) A gene responsible for cuticular hydrocarbon polymorphism in Drosophila melanogaster. Genet Res 73: 189–203.
[17]  Casares P (2007) Involvement of cuticular hydrocarbons in the mating success of Drosophila melanogaster females. Behav Genet 37: 498–506.
[18]  Grillet M, Dartevelle L, Ferveur J-F (2006) A Drosophila male pheromone affects female sexual receptivity. P Roy Soc B 273: 315–323.
[19]  Ferveur J-F, Sureau G (1996) Simultaneous influence on male courtship of stimulatory and inhibitory pheromones produced by live sex-mosaic Drosophila melanogaster. P Roy Soc B 263: 967–973.
[20]  Butterworth FM (1969) Lipids of Drosophila: a newly detected lipid in the male. Science 163: 1356–1357.
[21]  Brieger G, Butterworth FM (1970) Drosophila melanogaster: identity of male lipid in reproductive system. Science 167: 1262.
[22]  Jallon J-M, Antony C, Benamar O (1981) Un antiaphrodisiaque produit par les males de Drosophila melanogaster et transfere aux femelles lors de la copulation. C R Acad Sci Paris 292: 1147–1149.
[23]  Bartelt RJ, Schaner AM, Jackson LL (1985) Cis-vaccenyl acetate as an aggregation pheromone in Drosophila melanogaster. J Chem Ecol 11: 1747–1756.
[24]  Vander Meer RK, Obin MS, Zawistowski S, Sheehan KB, Richmond RC (1986) A reevaluation of the role of cis-vaccenyl acetate, cis-vaccenol, and esterase 6 in the regulation of mated female sexual attractiveness in Drosophila melanogaster. J Insect Physiol 32: 681–686.
[25]  Yew JY, Dreisewerd K, Luftmann H, Muthing J, Pohlentz G etal (2009) A new male sex-pheromone and novel cuticular cues for chemical communication in Drosophila. Curr Biol 19: 1245–1254.
[26]  Zawistowski S, Richmond RC (1986) Inhibition of courtship and mating of Drosophila melanogaster by the male-produced lipid cis-vaccenyl acetate. J Insect Physiol 32: 189–192.
[27]  Ejima A, Smith BPC, Lucas C, Van Naters WVG, Miller CJ, et al. (2007) Generalization of courtship learning in Drosophila is mediated by cis-vaccenyl acetate. Curr Bio 17: 599–605.
[28]  Scott D, Richmond RC (1987) Evidence against an antiaphrodisiac role for cis-vaccenyl acetate in Drosophila melanogaster. J Insect Physiol 33: 363–369.
[29]  Kurtovic A, Widmer A, Dickson BJ (2007) A single class of olfactory neurons mediates behavioural responses to a Drosophila sex pheromone. Nature 446: 542–546.
[30]  Yew JY, Cody RB, Kravitz EA (2008) Cuticular hydrocarbon analysis of an awake behaving fly using direct analysis in real-time time-of-flight mass spectrometry. P Natl Acad Sci U S A 105: 7135–7140.
[31]  Everaerts C, Farine J-P, Cobb M, Ferveur J-F (2010) Drosophila Cuticular Hydrocarbons Revisited: Mating Status Alters Cuticular Profiles. PLoS ONE 5(3): e9607. doi:10.1371/journal.pone.0009607.
[32]  Svetec N, Houot B, Ferveur , J-F (2005) Effect of genes, social experience, and their interaction on the courtship of transgenic Drosophila males. Genet Res Camb 85: 183–193.
[33]  Scott D (1986) Sexual mimicry regulates the attractiveness of Drosophila melanogaster females. P Natl Acad Sci U S A 83: 8429–8433.
[34]  Box GEP, Hunter WG, Hunter JS (1978) Statistics for Experimenters Wiley. New York: 653 p.
[35]  Petfield D, Chenoweth SF, Rundle HD, Blows MW (2005) Genetic variation in female condition predicts indirect genetic variance in male sexual display traits. P Natl Acad Sci U S A 102: 6045–6050.
[36]  Ritchie MG, Halsey EJ, Gleason JM (1999) Drosophila song as a species-specific mating signal and the bhavioural importance of Kyriacou and Hall cycles in D. melanogaster song. Anim Behav 58: 649–657.
[37]  Williams MA, Blouin AG, Noor MAF (2001) Courtship songs of Drosophila pseudoobscura and D. persimilis. II. Genetics of species differences. Heredity 86: 68–77.
[38]  Hoikkala A, Kappert , Mazzi D (2005) Factors affecting song evolution in Drosophila montana. Curr Top Dev Biol 67: 225–250.
[39]  Etges WJ, Oliviera CC, Gragg E, Ortiz-Barrientos D, Noor MAF (2007) Genetics of incipient speciation in Drosophila mojavensis. I. Male courtship song, mating success, and genotype X environment interactions. Evolution 61: 1106–1119.
[40]  Markow TA, Hanson SJ (1981) Multivariate analysis of Drosophila courtship. P Natl Acad Sci U S A 78: 430–434.
[41]  Blows MW (1999) Evolution of the genetic covariance between male and female components of mate recognition: an experimental test. P Roy Soc B 266: 2169–2174.

Full-Text

comments powered by Disqus