EXPRESIóN in vitro EN Escherichia coli, DE UN FRAGMENTO DEL GEN vlhA.5.02 DE LA PRINCIPAL FAMILIA DE HEMAGLUTININAS DE Mycoplasma gallisepticum

mycoplasma gallisepticum, the causal agent of the chronic respiratory disease of chickens, is the most economically significant for poultry industry. an 800pb dna fragment of mycoplasma gallisepticum which was amplified from the recombinant vector pttq18 was cloned into the expression vector pqe-32. the polypeptide was expressed as a fusion protein to 6x-histidine tag; hence its final size was 31kda. it was detected by western blott being recognized by a polyclonal antiserum to m. gallisepticum, as well as by a monoclonal antibody to histidine. the polypeptide was purified by nickel-nitrilotriacetic acid (ni-nta) metal-affinity cromatography yielding a final product of nearly 90% of purity.