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Detección y tipificación de virus del papiloma humano (VPH) mediante PCR- RFLP

Keywords: human papillomavirus, cervical infection, pcr-rflp, cervical cancer.

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Abstract:

objective: to standardize a pcr-rflp technique for detection and typification of hpv in women with clinical diagnostic of hpv infection. method: descriptive and transversal study with the assessment of 189 samples of the genital area of women that required dna extraction, diagnostic and typification for pcr-rflp molecular technique. setting: facultad de ciencias, laboratorio de biologia y medicina experimental labiomex, universidad de los andes. merida, estado merida, venezuela. results: the pcr-rflp was able to detect and identify the virus. the 16.8 % of the samples presented hpv of high types 16, 31, 33, 35, 56, 59 and 68; the 6.8 % presented hpv of intermediate risk types 51, 53, 58, 61 and 83; and 18 % the low risk types 6, 32, 53, 54, and 81. conclusions: the standardized pcr-rflp technique was appropriate for the diagnostic and typification of hpv in samples from the genital area. the 51.9 % of samples were positive to hpv, been the more common (20 %) the hpv high risk subtype 16, and the low risk hpv 6 the more frequent (23.8%).

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