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Toxicidad de un extracto tánico de Pinus caribaea Morelet

Keywords: pinus caribaea morelet, vegetable tanins, acute oral toxicity, acute dermal toxicity, rats, salmonella/microsome system, micronuclei, genotoxicity..

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Abstract:

acute oral, dermal and toxicogenetic toxicity of a spray dry-dried tanin powder from an aqueous extract of the wood of pinus caribaea morelet was determined. for all cases, wistar rats of both sexes weighing 150-200g were used. limit dose test and application of an occlusive patch on the skin for 24 hours determined the acute oral and dermal toxicities, respectively. the powder was administered at a dose of 2 000 mg/kg in both occasions. after 14 day-observation, the animals were killed to make autopsy and macroscopic test of organs and tissues. the toxicogenetic study was carried out in in vitro model called salmonella/microsome system(ames) and in vivo model, that is, the micronucleus induction test in the mouse bone marrow. in ames test, strains ta 100, ta 98, ta 1535 and ta 1537 of salmonella typhiumuriun with and without metabolic activation in the range of 50, 150, 500, 1 500 and 5 000 mg/plate concentrations were analyzed. doses of 500, 1 000 and 2 000 mg/kg of body weight were tested in the micronuclei induction test. it was proved that the spray dry-dried tanin powder obtained from an aqueous extract of the wood of pinus caribaea is not toxic by oral and dermal administration in the animals but it is genotoxic in vitro and in vivo. it will be advisable to perform other studies under different conditions so as to exactly determine the genotoxicity of this extract.

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