the degenerative joint disease, also known as osteoarthrosis affects to 10% of elderlies aged 60. it is mainly characterized by pain in the involved joint, crepitation, morning stiff and a progressive limitation of movement of that joint leading to a partial or total wear of articular cartilage. the treatment of the knee osteoarthrosis is a great challenge. the recent advances in use of regenerative medicine suggest that adult stem cells could represent a promisor alternative in the treatment of this entity. in a female patient aged 61 presenting with knee osteoarthrosis authors placed a percutaneous implant of autologous mononuclear cells mobilized to peripheral blood by granulocyte colony-stimulating factor achieving a fast clinical and radiological improvement. this result suggests that the procedure used is a feasible, simple, safe and less expensive method for treatment of articular degenerative lesions.