the paper shows the risks present in a sterilization center and their relation with biosafety. it identifies the different types of risks and makes a description of each of them. for obtaining information, observation was used as the primary data source and the existing records as a secondary source. the risks that may appear in a sterilization center are of biological, physical, chemical type and also those conditioned by human and environmental factors. the biological risk is the fundamental one. the purpose of this paper is to expand the level of knowledge and responsibility of the workers in a sterilization center so that they are better prepared for facing their daily work and preserving their health.