The long-tailed macaques (Macaca fascicularis) is a primate species widely used in ecology, socio-economics, and biomedical. Long-tailed macaques have a high degree of adaptation to various types of environments. This species can be used and traded as long as it is the product of captivity. Over time, with increasing demand, there is a tendency to increase the number of long-tailed macaques catch quotas from nature and reduce the habitat of these primates in Indonesia. This demand ...

Zoology  Conservation Biology 

Doi:10.29244/primatology.1.01.1-9

【Objective】 The purpose of this study was to investigate the toxic effect of perfuorooctanoic aid(PFOA) on mouse oocytes.【Method】 One hundred and sixty 6-week-old Kunming female mice with similar body weight were divided randomly into four groups, with five replicates in each group and eight mice in each replicate. After 7 days of adaptation, the mice were fed with different doses of PFOA for 14 days. The daily doses for control, low, medium, and high dose groups were 0, 5, 10 and 20 mg/kg respe...

Agricultural Science  Zoology  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202101028

【Objective】 To study the difference of protein modification of silkworm(Bombyx mori) eggs during diapause induced by high or low temperature, and provide references for further exploration of molecular mechanism of insect diapause induction. 【Method】 The stable diapause silkworm strains induced by high or low temperature were screened out by raising the bivoltine silkworm varieties. Using the selected strains as materials, the eggs were incubated at 25 and 15 ℃ respectively. Samples were taken a...

Agricultural Science  Zoology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202104013

【Objective】 Compared with the wild type mice, the homozygous ETV5 knockout mice prepared by CRISPR/Cas9 technology showed significant weakness in body size and body weight along with endogenous spermatogonial stem cell ablation. The purpose of this study was to explore the effect of ETV5 knockout on the muscle expression profile of mice. 【Method】 The muscle tissues of three wild-type male mice and three ETV5 homozygous knockout male mice aged six weeks were collected and total RNA was extracted ...

Agricultural Science  Zoology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202106023

【Objective】 To clarify serotype, drug resistance and genetic evolution of Rimerella anatipestifer in Guangdong. 【Method】 The isolates of R. anatipestifer were isolated and identified from samples of the large-scale duck farms. Serotype was identified by glass agglutination test. The minimum inhibitory concentration was tested by double dilution method in test tube, and the drug sensitivity was analyzed. Whole genome sequencing technology was used to analyze the sequence characteristices and cons...

Agricultural Science  Zoology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202105022

【Objective】 The purpose of this study was to investigate the effects of dietary supplementation of different conjugated linoleic acid (CLA) isomers on body fat, energy metabolism and gut microbiota in mice.【Method】 Mice were fed with normal diet (control group), normal diet supplemented with 2% cis-9, trans-11 conjugated linoleic acid (c9, t11-CLA) and trans-10, cis-12 conjugated linoleic acid (t10, c12-CLA) for 12 weeks respectively. The changes of fat content, energy metabolism and gut microbi...

Agricultural Science  Zoology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202110016

【Objective】 To investigate the effects of astragaloside IV (AS-IV) and astragalus polysaccharides (APS) of astragalus extract on the lipopolysaccharides (LPS)-induced inflammation of bovine mammary epithelial cells (BMECs) and the effect on the expression of β-casein in inflammatory cells. 【Method】 APS and AS-IV were used to intervene LPS-induced BMECs to detect the changes of cell inflammatory factors, oxidative factors, apoptosis factors and β-casein. 【Result】 CCK-8 screening found the concent...

Agricultural Science  Zoology  Cell Biology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202106034

【Objective】 Porcine 50K SNP (single nucleotide polymorphisms) chips have been widely used in pig genomic breeding. Meanwhile, genotype imputation can significantly increase the amount of genotype data without increasing the cost of sequencing, which facilitates genetic resolution and genetic evaluation of complex traits. This study was aimed to evaluate the genotype imputation performance from genotype to sequence data of three porcine SNP chips.【Method】 A total of 48 Duroc pigs with three kinds...

Agricultural Science  Zoology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202110032

【Objective】 To understand the community composition and diversity of birds in Qingyunshan Provincial Nature Reserve, Wengyuan, Guangdong.【Method】 In 2021, this study used line transect method to conduct field surveys of bird communities in four habitats of forest, highway, village, and reservoir in Qingyunshan Reserve, while interviewing local residents and reserve staff, looking up reserve basic information and scientific expedition reports, and searching for the records of China National Know...

Agricultural Science  Zoology  Agronomy 

Doi:10.7671/j.issn.1001-411X.202202009

【Objective】 To knock out the target chromosome by CRISPR/Cas9 system to cut multiple sites of Y chromosome, and provide a new method for sex control of livestock and poultry.【Method】 Based on CRISPR/Cas9 technology, we searched for multiple copies of repeat sequences on Y chromosome that can be specifically recognized by sgRNA, and verified their effectiveness on target by in vitro cleavage, quantitative analysis and karyotype identification.【Result】 The designed sgRNA could cut the target fragm...

Agricultural Science  Zoology 

Doi:10.7671/j.issn.1001-411X.202201022