All Title Author
Keywords Abstract

Publish in OALib Journal
ISSN: 2333-9721
APC: Only $99

ViewsDownloads

Relative Articles

More...

The Role of Sunscreen in Mitigating UV-Induced Damage in Saccharomyces cerevisiae Yeast Respiration and Survival, as a Eukaryotic Model Organism

DOI: 10.4236/abb.2024.1512044, PP. 696-723

Keywords: UV Radiation, Yeasts (Yeast Cells), Skin Cells, Cell Respiration (CO2 Rate, Cell Density)

Full-Text   Cite this paper   Add to My Lib

Abstract:

Mutagens are agents that cause damage to DNA and have the potential to permanently alter (mutate) its sequence, depending on the organism’s ability to repair the damage. UV radiation is a mutagen in cells. This mutagen relates to both yeast cells and human skin cells, since they have similar reactions. UV radiation can cause cell mutations, but also cell death. This is examined with the absence or presence of sunscreen when in contact with cells. Since yeast cells and human cells have almost identical metabolisms, data results of yeast experiments can be associated with real life. Hypothesis for the presence of sunscreen in yeast solutions includes the exposure of yeast cells with or without sunscreen for different time periods in UV radiation. However, the role of sunscreen in yeast cell mutations, in relation to cancer prevention, may not be directly positive. Here, I show that sunscreen has a positive effect on yeast cells and prevents mutations. I found that the respiration rate differs for yeast cells without or without the presence of sunscreen when exposed to radiation. Yeast cells without sunscreen respired faster than those exposed to UV radiation. However, with sunscreen, the rate of CO2 production was higher, with a higher respiration rate. These results may be connected with skin cancer to some extent, promoting or not the use of sunscreen to protect the skin cells from mutating. This experiment may be the base for further experimentation with different yeast cells, providing clearer and more assuring data about the association of sunscreen, yeast cells, and skin cancer. Such experiments may avoid implications with weather conditions, such as slightly different temperatures, sunlight intensity, and clouds, or with the time between the end of the time period of exposure of the yeast cells to UV radiation, and the measurement of CO2 and density, which my experiment had.

References

[1]  Baseadmin (2022). Where Is Yeast Found in Nature? Explore Yeast.
https://www.exploreyeast.com/what-is-yeast/where-is-yeast-found-in-nature/
[2]  Perkins, D.D. (2024) How to Use Auxanograms to Identify Nutritional Requirements.
https://www.fgsc.net/neurosporaprotocols/How to use auxanograms.pdf
[3]  Pincus, D.H., Orenga, S. and Chatellier, S. (2007) Yeast Identification—Past, Present, and Future Methods. Medical Mycology, 45, 97-121.
https://academic.oup.com/mmy/article/45/2/97/1216685
[4]  Chourpiliadis, C. and Mohiuddin, S.S. (2023) Biochemistry, Gluconeogenesis. StatPearls.
https://www.ncbi.nlm.nih.gov/books/NBK544346/
[5]  Glyoxylate Cycle.
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/glyoxylate-cycle
[6]  LibreTexts (2019) 2.4.03: Glycolysis.
https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/General_Biology_2e_(OpenStax)/02%3A_Unit_II-_The_Cell/2.04%3A_Cellular_Respiration/2.4.03%3A_Glycolysis
[7]  Green, A.C. and Williams, G.M. (2007) Point: Sunscreen Use Is a Safe and Effective Approach to Skin Cancer Prevention. Cancer Epidemiology, Biomarkers & Prevention, 16, 1921-1922.
https://doi.org/10.1158/1055-9965.epi-07-0477
[8]  Young, A.R., Claveau, J. and Rossi, A.B. (2017) Ultraviolet Radiation and the Skin: Photobiology and Sunscreen Photoprotection. Journal of the American Academy of Dermatology, 76, S100-S109.
https://doi.org/10.1016/j.jaad.2016.09.038
[9]  D’Orazio, J., Jarrett, S., Amaro-Ortiz, A. and Scott, T. (2013) UV Radiation and the Skin. International Journal of Molecular Sciences, 14, 12222–12248.
https://pmc.ncbi.nlm.nih.gov/articles/PMC3709783/#:~:text=Cutaneous%20Responses%20to%20UV&text=If%20the%20dose%20of%20UV,in%20epidermal%20thickness%2C%20termed%20hyperkeratosis
[10]  Matsumura, Y. and Ananthaswamy, H.N. (2004) Toxic Effects of Ultraviolet Radiation on the Skin. Toxicology and Applied Pharmacology, 195, 298-308.
https://doi.org/10.1016/j.taap.2003.08.019
[11]  Gensler, H.L. (1997) Prevention of Photoimmunosuppression and Photocarcinogenesis by Topical Nicotinamide. Nutrition and Cancer, 29, 157-162.
https://doi.org/10.1080/01635589709514618
[12]  Kockler, J., Oelgemöller, M., Robertson, S. and Glass, B.D. (2012) Photostability of Sunscreens. Journal of Photochemistry and Photobiology C: Photochemistry Reviews, 13, 91-110.
https://doi.org/10.1016/j.jphotochemrev.2011.12.001
[13]  Osmond, M.J. and Mccall, M.J. (2010) Zinc Oxide Nanoparticles in Modern Sunscreens: An Analysis of Potential Exposure and Hazard. Nanotoxicology, 4, 15-41.
https://doi.org/10.3109/17435390903502028
[14]  Antoniou, C., Kosmadaki, M., Stratigos, A. and Katsambas, A. (2008) Sunscreens—What’s Important to Know. Journal of the European Academy of Dermatology and Venereology, 22, 1110-1119.
https://doi.org/10.1111/j.1468-3083.2007.02580.x
[15]  Maier, T. and Korting, H.C. (2005) Sunscreens—Which and What for? Skin Pharmacology and Physiology, 18, 253-262.
https://doi.org/10.1159/000087606
[16]  Wulf, H.C. (2010) Sunscreens. In: Jemec, G.B.E., Kemeny, L. and Miech, D. Eds., Non-Surgical Treatment of Keratinocyte Skin Cancer, Springer, 167-176.
https://doi.org/10.1007/978-3-540-79341-0_21
[17]  Lazovich, D., Vogel, R.I., Berwick, M., Weinstock, M.A., Warshaw, E.M. and Anderson, K.E. (2011) Melanoma Risk in Relation to Use of Sunscreen or Other Sun Protection Methods. Cancer Epidemiology, Biomarkers & Prevention, 20, 2583-2593.
https://doi.org/10.1158/1055-9965.epi-11-0705
[18]  Gromkowska-Kępka, K.J., Puścion-Jakubik, A., Markiewicz-Żukowska, R. and Socha, K. (2021) The Impact of Ultraviolet Radiation on Skin Photoaging—Review of in vitro Studies. Journal of Cosmetic Dermatology, 20, 3427-3431.
https://pmc.ncbi.nlm.nih.gov/articles/PMC8597149/#:~:text=Changes%20arising%20as%20a%20result,the%20skin%20and%20precancerous%20lesions
https://doi.org/10.1111/jocd.14033
[19]  American Cancer Society (n.d.) Cytokines and Their Side Effects.
https://www.cancer.org/cancer/managing-cancer/treatment-types/immunotherapy/cytokines.html#:~:text=Cytokines%20are%20small%20proteins%20that,body's%20immune%20and%20inflammation%20responses
[20]  Pfeifer, G.P. (2020) Mechanisms of UV-Induced Mutations and Skin Cancer. Genome Instability & Disease, 1, 99-113.
https://doi.org/10.1007/s42764-020-00009-8
[21]  Waris, G. and Ahsan, H. (2006) Reactive Oxygen Species: Role in the Development of Cancer and Various Chronic Conditions. Journal of Carcinogenesis, 5, Article No. 14.
https://doi.org/10.1186/1477-3163-5-14
[22]  Ingredients Explained (n.d.) Frezyderm Seaside Dry Mist.
https://incidecoder.com/products/frezyderm-sea-side-dry-mist
[23]  Luedeking, R. and Piret, E.L. (1959) A Kinetic Study of the Lactic Acid Fermentation. Batch Process at Controlled pH. Journal of Biochemical and Microbiological Technology and Engineering, 1, 393-412.
https://doi.org/10.1002/jbmte.390010406
[24]  Proshlyakov, D.A. (2004) UV Optical Absorption by Protein Radicals in Cytochrome c Oxidase. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1655, 282-289.
https://doi.org/10.1016/j.bbabio.2003.10.014
[25]  Sun, J., Jeffryes, J.G., Henry, C.S., Bruner, S.D. and Hanson, A.D. (2017) Metabolite Damage and Repair in Metabolic Engineering Design. Metabolic Engineering, 44, 150-159.
https://doi.org/10.1016/j.ymben.2017.10.006
[26]  Xue, F., Dong, H., Wu, J., Wu, Z., Hu, W., Sun, A., Troxell, B., Yang, X.F. and Yan, J. (2010) Transcriptional Responses of Leptospira interrogans to Host Innate Immunity: Significant Changes in Metabolism, Oxygen Tolerance, and Outer Membrane. PLOS Neglected Tropical Diseases, 4, e857.
https://doi.org/10.1371/journal.pntd.0000857
[27]  Bedford, J.S. (1991) Sublethal Damage, Potentially Lethal Damage, and Chromosomal Aberrations in Mammalian Cells Exposed to Ionizing Radiations. International Journal of Radiation OncologyBiologyPhysics, 21, 1457-1469.
https://doi.org/10.1016/0360-3016(91)90320-4
[28]  Seshadri, P.R., Balakrishnan, B., Ilangovan, K., Sureshbabu, R. and Mohanakrishnan, A.K. (2009) Ethyl 2-[N-(2-Formylphenyl)Benzenesulfonamido]Acetate. Acta Crystallographica Section E Structure Reports Online, 65, o530.
https://doi.org/10.1107/s1600536809004413
[29]  Fontana, R.J. (2010) Approaches to the Study of Drug-Induced Liver Injury. Clinical Pharmacology and Therapeutics, 88, 416-419.
https://doi.org/10.1038/clpt.2010.100
[30]  Apoptosis.
https://www.genome.gov/genetics-glossary/apoptosis#:~:text=Apoptosis%20is%20the%20process%20of,have%20been%20damaged%20beyond%20repair
[31]  Lomax, M.E., Folkes, L.K. and O’Neill, P. (2013) Biological Consequences of Radiation-Induced DNA Damage: Relevance to Radiotherapy. Clinical Oncology (Royal College of Radiologists (Great Britain)), 25, 578-585.
https://doi.org/10.1016/j.clon.2013.06.007
[32]  Van Thomme, G. and Blackburn, K.B. (2024) How Does Sunscreen Work? Understanding UV Protection.
https://www.mdanderson.org/cancerwise/how-does-sunscreen-work.h00-159698334.html#:~:text=Chemical%20sunscreen%20ingredients%20form%20a,ingredients%20have%20pros%20and%20cons
[33]  Sunscreen in the Environment.
https://ocean.si.edu/ecosystems/coral-reefs/sunscreen-environment#:~:text=Coral%20reefs%20are%20particularly%20susceptible,ingredients%20like%20oxybenzone%20and%20octinoxate
[34]  Ethylhexyl Methoxycinnamate.
https://www.aako.nl/products/sun-care/ethylhexyl-methoxycinnamate/#:~:text=Ethylhexyl%20Methox-ycinnamate%20is%20organic%2C%20oil,a%20peak%20protection%20at%20310nm
[35]  Ataman Kimya (n.d.) Diethylamino Hydroxybenzoyl Hexyl Benzoate.
https://www.atamanchemicals.com/diethylamino-hydroxybenzoyl-hexyl-benzoate_u25308/
[36]  Octocrylene.
https://www.sciencedirect.com/topics/medicine-and-dentistry/octocrylene
[37]  Ataman Kimya (n.d.) Ethylhexyl Salicylate.
https://www.atamanchemicals.com/ethylhexyl-salicylate_u26075/#:~:text=Ethylhexyl%20salicylates%20are%20weak%20UVB,peak%20at%20307%2D310%20nm
[38]  COSMILE Europe (2023) Ingredient.
https://cosmileeurope.eu/inci/detail/2000/butyl-methoxydibenzoylmethane/

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133