This study aimed to compare the total phenolic
content (TPC) and antioxidant activities of A.rigidula extracts. It also aimed to
identify phenolic acids present in the extracts. The
2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS),
2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP),
and ferric thiocyanate lipid peroxidation antioxidant assays were performed.
High performance liquid chromatography was used to identify phenolic acids.
There was no solvent effect on TPC nor on scavenging
activities, and inhibition of lipid peroxidation (p > 0.05) among solvent
extracts. On the other hand, 1:1:3 water: acetone: methanol extract (10.22 mg
GAE/g sample) had significantly higher reducing potential than 50% ethanol
extract (EE) (9.259 mg GAE/g sample) (p < 0.05); but EE was not
significantly different from 80% methanol extract (9.781 mg GAE/g sample) (p
> 0.05). Phenolic fraction designated as fraction 4 had the highest
antioxidant activity (p < 0.05) with 69.49% ABTS scavenging activity and
FRAP reducing potential, 22.26 mg of GAE/g
sample. DPPH scavenging activities of fractions 4 (55.59%) and 5
(55.64%) were significantly higher than the other fractions (p < 0.05). A.rigidula extracts contain gallic, caffeic, vanillic, p-coumaric, salicylic acids and
vanillin.
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