Biosynthesis, Characterization and Application of Chitin Nanoparticle with Cassia auriculata

Prawns are a common food for humans all around the World. Particularly the prawn exists naturally in south East Asia. Prawn shells contain ash, fibre, minerals and proteins. Prawns like a Shrimps. It’s a Dendrobranchiata family. Prawn shells were collected from Chindaripet market. Deproteinization followed by demineratisation was carried out to obtain the final product chitin. Cassia auriculata is a legume tree in the subfamily Caesalpinioideae. It contains antioxidants, cardiac Glycosides, anthraguinones, tannins, carbohydrates, alkaloids, phenols, saponin and flavonoids. Cassia auriculata flower has anti- bacterial, anti- diabetic property, the flower taste is stringent, pungent and bitter. Cassia auriculata flowers were dried under room temperature for 2 to 3weeks flowers and grounded with mechanical blender. Then chitin and silver nanoparticles were prepared along with Cassia auriculata flower extract which was extracted with both ethanol and water. Scanning Electron Microscope (SEM) was done by chitin nanoparticles to identify the particle size and morphology. Fourier Transform Infrared Spectroscopy (FTIR) was done to identify the active compounds. Chitin nanoparticles were used widely in various applications that evaluates anti-microbial activity using E. coli, Staphylococcusaureus, Salmonella typhi, Klebsiella pneumoniae, Staphylococcus mutans, Entero coccus spp, then anti-inflammatory activity gives better inhibition, anti-larvae activity using mosquito larvae. Cassia auriculata (fresh, ethanol) nanoparticles killed all larvae, anti-coagulant and thrombolytic activity were observed