Experimental investigations of submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus Niger : Effect of inoculum size and age of spores

The paper investigates effects of the different concentration of inoculums and age of spores on the synthesis of pectinolytic enzymes with the aim of their optimizing for maximal enzyme production and growth of the microorganism. The microorganism used in this work was the fungus Aspergillus niger MK-15, which was isolated from soil as a highly active producer of pectinolytic enzymes. The results showed that the concentration of the inoculum and age of spores have a pronounced effect on the pectinolytic activity and dry weight. Obtained results suggest that for submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus niger MK-15, the optimal inoculum concentration is 3% or 6x106 spores and optimal age of spores is 3 days old.