Binary Combination of Different Breeds of Freeze-Dried Cow Urine (FCU) with Some Plant Molluscicides against Lymnaea acuminata: Vector of Fasciolosis

Molluscicidal activity of binary combination of different breeds of freeze-dried cow urine (FCU) such as Sahiwal, Geer and Tharparkar with plant molluscicides viz Annona squamosa seed powder, Azadirachta indica oil, Ferula asafoetida root latex and Camellia sinensis against harmful snail Lymnaea acuminata have been studied. It was observed that the toxicity of binary combination (1:1) of plant molluscicides and different breeds of freeze-dried cow urine (FCU) kept for 15 days in sunlight (8h/day) and laboratory condition were toxic against L. acuminata. Among all combinations, the toxicity of Sahiwal freeze-dried cow urine + A. squamosa (24h LC50 -97.27mg/l and 96h LC50 -36.19mg/l) kept for 15 days in sunlight (8h/day) was higher than the Geer and Tharparkar freeze-dried cow urine. Toxicity of laboratory condition of Sahiwal freeze-dried cow urine + A. squamosa (24h LC50 -81.40mg/l and 96h LC50 -34.30mg/l) was more toxic than other binary combination of cow breeds and plants molluscicides. The different breeds of cow urine under the 15 days sunlight (8h/day) and laboratory conditions freeze-dried with different plant molluscicides are potent molluscicides.