The use of ferromagnetic dacron as solid-phase in enzyme immunoassays

ferromagnetic dacron is proposed as an alternative solid-phase for magnetic enzyme immunoassays. human serum albumin (hsa) was covalentlyimmobilized onto ferromagnetic dacron and as enzyme immunoassay was developed using anti-hsa rabbit sera. peroxidase, o-phenylenediamine (opd) and hydrogen peroxide were used anti-hsa rabbit sera. peroxidase, o-phenylenediamine (opd) and hydrogen peroxide were used as the enzymatic label and substrates, respectively. best results were observed when particles of 63-100 μm (diameter) and 10 μg of immobilized antigen were used. positive reactions were detected until dilutions of1:51200 of immune sera. its reproducibility was similar to standard elisa. disruption of the immunocomplexes formed and recuperation of the immobilized antigen in other immunoassays also proved to be reliable.