Descondensación de la heterocromatina en bovinos criollos portadores de la translocación Robertsoniana (rob1; 29). Acción del inductor 5-azacitidina-C

the robertsonian translocation (rob1; 29) is widely spread in commercial breeds and specially in genetic reserve of american creole cattle. it is also described a delay on embryo development in front of normal ones. the action of 5-aza-c, as an hypomethylated agent, could permitted to decondensate the constitutive or facultative heterochromatin. in this work we made induction with the 5-aza-c(10mm, 2 hrs) analogs, in lymphocyte cultures of female and male carriers and normal creole cattle. the dna hypometilation is found in the inactive x chromosome of late replication as it is incorporated during the last hours of cell culture. the decondensing effects of 5-aza-c analogs is observed in multiple regions of the autosomes chromatin, the rob1; 29 and the bta1. a correlation between hypometilated regions and decondensed of facultative (conditional) heterochromatin is related with genomic instability, and epigenetic reprogramming.