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Acta Agronómica 2008
Caracterización molecular de un begomovirus del tomate en el Valle del Cauca, Colombia, y búsqueda de fuentes de resistencia para el mejoramiento de la variedad Unapal MaravillaKeywords: solanum lycopersicum, bemisia tabaci, toymv. Abstract: a virus transmitted by the whitefly bemisia tabaci to tomato was characterized in the cauca valley like a variant of tomato yellow mosaic virus (toymv). artificial whitefly-mediated inoculation in the greenhouse was done with 20 days-old tomato plants (fla 496-11-6-1-0, fla 478-6-3-1-11, fla 456-4 y fla 653-3-1-0) exposed to 10 viruliferous individuals of b. tabaci (biotype b) per plant in individual insect-proof cages. the presence of the begomovirus was evaluated by symptoms development and was confirmed using dot blot hybridization and pcr. agronomical characteristics were evaluated in the field in a completely randomized blocks design with 3 replications. the lines fla 653-3-1-0, fla 496-11-6-1-0 and fla 478-6-3-1-11 developed mild symptoms, viral dna was barely detectable in some individuals, and they showed characteristics of the fruit and desirable yield.
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