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编码疾病相关的固有无序蛋白同义密码子使用偏好性的研究
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Abstract:
固有无序蛋白(简称IDPs)在生理条件下不具有稳定的空间结构，但是在生物体内发挥重要的生物学功能，除此之外，尤为重要的是它们与人类许多重大疾病密切相关。因此，研究疾病相关的IDPs，可以进一步了解IDPs与一系列疾病的病理学上的关系，这为蛋白质的药物设计和疾病治疗提供新思路。研究表明：密码子的使用偏好与固有无序蛋白的无序程度存在一定的关联性。本文研究了编码疾病相关的固有无序蛋白(IDPs)中同义密码子和GC含量的使用偏好，以及编码这些蛋白质的有序/无序区域的密码子中四个核苷酸在密码子的三个位点的分布差异。结果表明，这些蛋白中同义密码子的使用、GC含量均存在显著差异，另外四个核苷酸在其密码子三个位点的分布在编码3类疾病相关的IDPs有序和无序区中均存在偏好性。这些结果为后期研究IDPs提供了很好的参考信息。
Despite the fact that intrinsically disordered proteins (IDPs) lack stable spatial structures under physiological conditions, they perform critical biological functions in organisms. Moreover, intrinsically disordered proteins (IDPs) are associated with many major human diseases. Therefore, the systematic study of disease-associated intrinsically disordered proteins (IDPs) can further understand the pathological relationship between intrinsically disordered proteins (IDPs) and a series of diseases, providing a new idea for protein drug design and disease treatment. Some studies have shown a specific correlation between codon usage bias and intrinsically disordered proteins (IDPs) or intrinsically disordered regions (IDRs). In this paper, we analyzed the use bias of synonymous codons and GC content in nucleic acids encoding the disease-associated intrinsically disordered proteins (IDPs). Moreover, we studied the distribution of four nucleotides at three sites of the codon in encoding the ordered/disordered regions of these proteins. The results showed that the use of synonymous codons, the content of GC, and the distribution of four nucleotides at three sites of the codon are biased in the encoding of ordered regions and disordered regions of disease-associated intrinsically disordered proteins (IDPs). The results can provide a reference for further study of intrinsically disordered proteins (IDPs) or intrinsically disordered regions (IDRs).
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