LCMS检测维生素的室内质控血清制备及可行性研究
Preparation and Feasibility Study of Internal Quality Control Serum for Vitamins Detection in LCMS

目的：研制稳定的维生素质控血清，用于实验室内部维生素检测的质量控制。方法：准确称取一定量的维生素A、25-羟基维生素D₂、25-羟基维生素D₃、维生素E和维生素K₁溶于适量的二甲基亚砜中成为母液，适量添加至基质血清中，形成高、低质控血清。分装成1 ml/支，分别贮存在4℃、？20℃和？80℃环境中，以观察其在不同贮存条件下的稳定性和均一性。结果：4℃保存的质控血清，从第4天起维生素K₁值迅速下降，其他维生素(A、D₂、D₃和E)在一周后检测值也明显下降，？20℃和？80℃保存的质控血清中几种维生素的检测值都均可稳定1年以上；均一性检测表明样品间无显著性差异(F值 < F_0.05)，均一性良好。结论：本研究制备的质控血清具有良好的均一性和稳定性，可用于实验室内部维生素检测的质量控制。
Objective: To develop the stable vitamin quality control serum for the quality control of vitamin testing in the laboratory. Methods: A certain amount of vitamin A, 25-hydroxyvitamin D₂, 25- hydroxyvitamin D₃, vitamin E and vitamin K1 was dissolved in the appropriate dimethyl sulfoxide to form mother liquor and added to the matrix serum into high- and low-quality control serum. Repacked into 1 ml/bottle and stored in 4？C, ？20？C, and ？80？C environments to observe their stability and uniformity under different storage conditions. Results: In 4？C environments, concentration of vitamin K1 in the quality control serum was decreased rapidly from day 4, and other vitamins (A, D₂, D₃ and E) did so after a week. The detection values of several vitamins in the quality control serum in ？20？C and ？80？C were stable for more than 1 year; uniformity tests showed no significant difference between samples (F value < F _0.05), the results showed good uniformity. Conclusion: The quality control serum prepared in this study has good uniformity and stability, and can be used for the quality control of internal laboratory vitamin testing.