|
|
PTEN及Villin与早期胃癌诊疗的相关性研究
|
Abstract:
目的：胃腺癌作为常见癌症之一，虽然目前其诊疗方案日趋成熟，但其早期诊断及预后判断仍待完善。方法：本实验利用TCGA数据库筛选出胃腺癌潜在致病基因，并筛选相关hub基因，并做生存曲线。最后利用组织样本对上述结果进行验证。结果：利用TCGA数据库共筛选出29个潜在胃腺癌致病靶点，并筛选出10个关键基因。对关键基因进行数据分析，发现PTEN低表达及Villin高表达与胃癌患者生存率下降相关，最后利用免疫组化对现有标本进行验证。结论：PTEN低表达及Villin高表达可作为早期胃腺癌诊断的重要参考，其可能影响胃腺癌患者的预后。
Objective: Stomach adenocarcinoma is one of common cancer. Although its diagnosis and treatment plan is increasing, its early diagnosis and prognosis judgment remain treated. Methods: This experiment utilizes TCGA database to screen a potential pathogenic gene of gastric adenocarcinoma and screen the related HUB genes, and do survival curves. Finally, the above results are verified by tissue samples. Results: 29 potential gastric adenocarcinomas were screened using TCGA databases, and ten key genes were screened. Data analysis of key genes found that PTEN low expression and Villin high expression were related to the decrease in the survival rate of patients with gastric cancer. Finally, the existing specimens were verified using immunohistochemistry. Conclusion: PTEN low expression and Villin high expression can be an important reference for the diagnosis of early gastric adenocarcinoma, which may affect the prognosis of patients with gastric adenocarcinoma.
| [1] | Yao, Q., He, L., Gao, X., Tang, N., Lin, L., Yu, X., et al. (2021) The m6A Methyltransferase METTL14-Mediated N6-Methyladenosine Modification of PTEN mRNA Inhibits Tumor Growth and Metastasis in Stomach Adenocarcinoma. Frontiers in Oncology, 11, Article ID: 699749. https://doi.org/10.3389/fonc.2021.699749 |
| [2] | Yang, Y., Li, Y. and Du, X. (2021) Prognostic Analysis of Gastric Signet Ring Cell Carcinoma and Hepatoid Adenocarcinoma of the Stomach: A Propensity Score-Matched Study. Frontiers in Oncology, 11, Article ID: 716962.
https://doi.org/10.3389/fonc.2021.716962 |
| [3] | Liu, Z., Wang, A., Pu, Y., Li, Z., Xue, R., Zhang, C., et al. (2021) Genomic and Transcriptomic Profiling of Hepatoid Adenocarcinoma of the Stomach. Oncogene, 40, 5705-5717. https://doi.org/10.1038/s41388-021-01976-2 |
| [4] | Han, S., Zhu, W., Yang, W., Guan, Q., Chen, C., He, Q., et al. (2021) A Prognostic Signature Constructed by CTHRC1 and LRFN4 in Stomach Adenocarcinoma. Frontiers in Genetics, 12, Article ID: 646818.
https://doi.org/10.3389/fgene.2021.646818 |
| [5] | Hempel, L., De Oliveira, J.V., Gaumann, A., Milani, V., Schweneker, K., Schenck, K., et al. (2021) Landscape of Biomarkers and Actionable Gene Alterations in Adenocarcinoma of GEJ and Stomach-A Real World Data Analysis. Cancers, 13, Article No. 4453. https://doi.org/10.3390/cancers13174453 |
| [6] | Guo, Y., Zhou, K., Zhuang, X., Li, J. and Shen, X. (2021) CDCA7-Regulated Inflammatory Mechanism through TLR4/NF-κB Signaling Pathway in Stomach Adenocarcinoma. BioFactors. https://doi.org/10.1002/biof.1773 |
| [7] | 李玉龙, 柴宁莉, 令狐恩强, 等. 预测老年早期胃癌患者内镜黏膜下剥离术后复发风险的列线图模型[J]. 中华老年医学杂志, 2021, 40(2): 188-192. https://doi.org/10.3760/cma.j.issn.0254-9026.2021.02.011 |
| [8] | 贾音, 孙婷婷, 刘海东, 等. 常规检验大数据在胃癌早期诊断中的应用[J]. 中华检验医学杂志, 2021, 44(3): 197-203. |
| [9] | Wu, S., Cao, L., Ke, L., Yan, Y., Luo, H., Hu, X., et al. (2021) Knockdown of CENPK Inhibits Cell Growth and Facilitates Apoptosis via PTEN-PI3K-AKT Signalling Pathway in Gastric Cancer. Journal of Cellular and Molecular Medicine, 25, 8890-8903. https://doi.org/10.1111/jcmm.16850 |
| [10] | Fan, J.P., Qian, J. and Zhao, Y.J. (2020) The Loss of PTEN Expression and Microsatellite Stability (MSS) Were Predictors of Unfavorable Prognosis in Gastric Cancer (GC). Neoplasma, 67, 1359-1366.
https://doi.org/10.4149/neo_2020_200422N427 |
| [11] | Langdon, C.G., Gadek, K.E., Garcia, M.R., Evans, M.K., Reed, K.B., Bush, M., et al. (2021) Synthetic Essentiality between PTEN and Core Dependency Factor PAX7 Dictates Rhabdomyosarcoma Identity. Nature Communications, 12, Article No. 5520. https://doi.org/10.1038/s41467-021-25829-4 |
| [12] | Hernandez-Llodra, S., Segales, L., Juanpere, N., Marta Lorenzo, T., Salido, M., Nonell, L., et al. (2021) SPOP and CHD1 Alterations in Prostate Cancer: Relationship with PTEN Loss, Tumor Grade, Perineural Infiltration, and PSA Recurrence. Prostate. https://doi.org/10.1002/pros.24218 |
| [13] | 罗文, 周娟, 夏红, 苏坚, 刘芳, 汤欢, 等. PTEN蛋白在胃癌中的表达及临床病理意义[J]. 中南医学科学杂志, 2017, 45(2): 109-112, 144. https://doi.org/10.15972/j.cnki.43-1509/r.2017.02.001 |
| [14] | 甘伟平, 罗陈飘, 陈喜志, 吴东海, 王立生. 抑癌基因PTEN和p53在胃癌中的表达及意义[J]. 中华实用诊断与治疗杂志, 2016, 30(1): 68-70. https://doi.org/10.13507/j.issn.1674-3474.2016.01.023 |
| [15] | Zhao, L., Liu, S.Y., Li, Y.M. and Xiong, Z.T. (2021) Villin Is a Biomarker for Reverse Polarity in Colorectal Micropapillary Carcinoma. Oncology Letters, 21, Article No. 72. https://doi.org/10.3892/ol.2020.12333 |
| [16] | Nasser, M.E.T., Abou Elleil, H.A., Abdel Mohsen, W.A., Ali Ezzat, M. and Mohamed Teama, N. (2021) Value of Plasmatic Villin-1 in the Prediction of Early Graft Dysfunction in Kidney Transplant Recipients: Diagnostic Accuracy Test. Experimental and Clinical Transplantation, 19, 110-117. https://doi.org/10.6002/ect.2020.0160 |
| [17] | 王见璋, 李炜霞, 何志明, 黄金凤. CDX2和Villin在胃黏膜病变中的表达及其临床病理意义[J]. 临床与病理杂志, 2017, 37(9): 1821-1824. https://doi.org/10.3978/j.issn.2095-6959.2017.09.009 |
