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Botanical Research 2021
利用转录组数据分析水稻植株包穗的分子机制
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Abstract:
包穗会引起水稻不能正常授粉，进而降低水稻的产量和经济效益。研究水稻抽穗的调控机理具有非常重要的科学意义和应用价值。CRKs (CDPK-related kinases)是一种植物特有的钙依赖性蛋白激酶相关激酶。水稻CRK5-RNAi植株在抽穗期部分或全部稻穗被顶叶鞘包裹。本文以RNAi植株和野生型水稻抽穗期间的倒一节部位为实验材料，利用RNA-Seq技术探索研究水稻植株包穗的机制。结果显示，RNAi植株中2581个基因表达上调，2208个下调；GO分析和KEGG分析表明DEGs主要富集在植物激素信号转导、代谢过程、催化活性等。qRT-PCR实验表明ABA应答基因、GA信号通路基因、转录因子和叶绿体合成相关基因等10个DEGs表达趋势与RNA-Seq一致，表明CRK5基因在抽穗期间主要在植物激素信号转导通路中发挥功能。
Panicle enclosure can cause the failure of rice pollination, and then reduce the yield and economic benefit of rice. It is of great scientific significance and application value to study the regulation mechanism of rice heading. CRKs (CDPK-related kinases) is a plant-specific calcium-dependent protein kinase-related kinase. In rice CRK5-RNAi plants, part or all of the panicles are covered by apical leaf sheaths at the heading stage. In this study, RNAi plants and the inverted portion of wild-type rice during heading were used as experimental materials to explore the mechanism of rice panicle enclosure by RNA-Seq technology. The results showed that 2581 genes were up-regulated and 2208 genes down-regulated in RNAi plants. GO analysis and KEGG analysis showed that DEGs were mainly enriched in plant hormone signal transduction, metabolic process and catalytic activity. qRT-PCR showed that the expression trends of 10 DEGs, including ABA response genes, GA signaling pathway genes, transcription factors and chloroplast synthesis genes, were consistent with RNA-Seq, indicating that CRK5 gene mainly plays a role in plant hormone signal transduction pathway during heading.
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