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基于纳米生物技术的三明治结构在蛋白质免疫分析中研究进展
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Abstract:
蛋白质组研究在重大疾病机制以及药理控制机制探索上有着重要的意义，通过对生物样品(细胞、组织或体液等)在生理过程中蛋白质含量的比较分析，可以解码疾病模式与蛋白质组间的未知关联。近年来基于三明治结构的蛋白质检测技术已成为热门的研究方向。构建操作简便、快速灵敏的新型蛋白质生物传感器对生物医学的基础性研究，以及疾病的早期/预后临床诊断不但意义重大，而且切实可行。本文首先简要阐述了三明治免疫分析的基本原理，随后按不同读出方法，包括放射、比色、荧光、电化学以及局域表面等离子体共振等方面对其近年来的发展进行了回顾，主要集中在基于纳米生物技术的三明治结构在蛋白质分析的应用上。
Proteome research is of great significance in the exploration of major disease mechanisms and pharmacological control mechanisms. Through the comparative analysis of protein content in biological samples (cells, tissues or body fluids, etc.) in physiological processes, the unknown relationship between disease patterns and proteome can be decoded. In recent years, protein detection technology based on sandwich structure has become a hot research direction. The construction of a simple, rapid and sensitive new protein biosensor is not only of great significance, but also practical for the basic research of biomedicine and the early/prognostic clinical diagnosis of diseases. This paper first briefly describes the basic principle of sandwich immunoassay, and then reviews the development in recent years according to different readout methods, including radiation, colorimetry, fluorescence, electrochemistry and local surface plasmon resonance, mainly focusing on the application of sandwich structure based on nano biotechnology in protein analysis.
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