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羊肚菌多糖对人结肠癌细胞SW620体外增殖的抑制作用
Inhibitory Effect of Morchella Polysaccharide on the Proliferation of Human Colon Cancer Cell Line SW620 in Vitro

DOI: 10.12677/HJFNS.2021.103023, PP. 199-205

Keywords: 羊肚菌多糖，结肠癌细胞，抑制作用
Morchella Polysaccharide, Colon Cancer Cell, Inhibition

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Abstract:

目的：研究羊肚菌多糖对人结肠癌细胞SW620体外增殖的抑制作用。方法：采用mtt法、划线法和集落生成分析法检测羊肚菌多糖对人结肠癌细胞SW620的体外增殖抑制作用，采用流式细胞技术检测羊肚菌多糖对人结肠癌细胞SW620的凋亡作用，采用western blotting技术检测相关凋亡蛋白的表达情况。结果：1) 羊肚菌多糖对人结肠癌细胞SW620的体外增殖、细胞迁移、细胞集落形成具有抑制作用，其作用24 h的IC50为1220 ± 50 μg/mL，48 h的IC50为968 ± 63 μg/mL；2) 羊肚菌多糖通过凋亡途径抑制SW620细胞的增殖；3) 在羊肚菌多糖的作用下抗凋亡蛋白Bcl-2的表达下降，而凋亡蛋白Bax和caspase-3的表达上升。结论：羊肚菌多糖能显著抑制人结肠癌细胞SW620的增殖，并通过上调Bax和caspaes-3的表达和下调Bcl-2的表达诱导SW620细胞凋亡。
Objective: To study the inhibitory effect of Morchella polysaccharide on human colon cancer cell line SW620 in vitro. Methods: MTT assay, scratch wound-healing and colony-forming analysis were used to detect the inhibitory effect of Morchella polysaccharide on the proliferation of human colon cancer cell SW620 in vitro, flow cytometry was used to detect the apoptosis effect of Morchella polysaccharide on human colon cancer cell SW620, and western blotting was used to detect the expression of related apoptosis proteins. Results: 1) Morchella polysaccharide treatment resulted in inhibition of proliferation, migration and colony formation of SW620 cancer cell in a time and dose-dependent, and the IC50 of Morchella polysaccharide is 1220 ± 50 μg/mL for 24h and 968 ± 63 μg/mL for 48 h; 2) Morchella polysaccharide inhibits the proliferation of SW620 cells through the apoptotic pathway; 3) Morchella polysaccharide treatment-induced SW620 cancer cell apoptosis through up-regulated the expression of caspase-3 and Bax and down-regulated Bcl-2 levels. Conclusion: Morchella

References

[1]  Bray, F., Ferlay, J., Soerjomataram, I., et al. (2018) Lobal Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians, 68, 394-424.
https://doi.org/10.3322/caac.21492
[2]  朱林, 程显好, 田吉藤. 羊肚菌研究进展安[J]. 安徽农业科学, 2008, 36(10): 4054-4057.
[3]  张利平, 陈彦, 王子尧. 羊肚菌胞外多糖免疫活性研究[J]. 中国食用菌, 2009, 28(3): 47-49.
[4]  崔华丽. 羊肚菌多糖的结构特点和抗肿瘤机制的研究[D]: [硕士学位论文]. 合肥: 安徽大学, 2011.
[5]  刘超. 液体深层发酵羊肚菌胞内多糖提取、结构分析及抗结肠癌作用研究[D]: [博士学位论文]. 长春: 吉林大学, 2017.
[6]  梁宇芝, 王清爽, 王一东. 羊肚菌多糖应急性抗疲劳成分的初步分离与鉴定[J]. 长春理工大学学报(自然科学版), 2020, 43(3): 130-135.
[7]  Shiozaki, A., Bai, X., Shen-Tu, G., Moodley, S., Takeshita, H., et al. (2012) Claudin 1 Mediates TNFa-Induced Gene Expression and Cell Migration in Human Lung Carcinoma Cells. PLoS ONE, 7, e38049.
https://doi.org/10.1371/journal.pone.0038049
[8]  Gao, W., Zhang, X.Y., Jiang, R., et al. (2011) Lentivi-rus-Mediated RNA Interference and Over-Expression of Over-Expression of CDKA2AP1 cDNA Regulate CDKA2AP1 Expression in Human Lung Cancer A549 Cells. Chemical Research in Chinese Universities, 27, 445-449.
[9]  Li, Q., Zhou, S., Jing, J., et al. (2013) Oligosaccharide from Apple Induces Apoptosis and Cell Cycle Arrest in HT29 Human Colon Cancer Cells. International Journal of Biological Macromolecules, 57, 245-254.
https://doi.org/10.1016/j.ijbiomac.2013.03.034
[10]  陈义勇. 桦褐孔菌多糖纯化、结构及其抗肿瘤机制研究[D]: [硕士学位论文]. 无锡: 江南大学, 2010.
[11]  Adams, J.M. and Cory, S. (2007) The Bcl-2 Apoptotic Switch in Can-cer Development and Therapy. Oncogene, 26, 1324-1337.
https://doi.org/10.1038/sj.onc.1210220
[12]  Mallat, Z. and Tedgui, A. (2000) Apoptosis in the Vasculature: Mechanisms and Functional Importance. British Journal of Phar-macology, 130, 947-962.
https://doi.org/10.1038/sj.bjp.0703407

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