利用微滴式数字PCR技术检测初榨菜籽油中的转基因成分
Detection of Genetically Modified Virgin Colza Oil by Droplet Digital PCR

DOI: 10.12677/HJAS.2021.117086, PP. 622-629

Keywords: 初榨菜籽油，转基因，DNA，微滴式数字PCR
Virgin Rapeseed Oil, Transgene, DNA, Droplet Digital PCR

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Abstract:

我国是食用植物油脂、油料进口大国，鉴于全球转基因油菜种植现状，由转基因油菜籽加工而成的菜籽油已经成为了消费者生活中的食用油，判定食用的菜籽油是否含有转基因成分，是国家监管部门和公众消费者密切关注的问题。食用植物油转基因成分检测技术长期以来受限于油脂中的核酸分离纯化，目前为止暂无现行有效的检测标准。本研究同时采用实时荧光PCR技术和微滴式数字PCR (ddPCR)技术检测初榨菜籽油中的外源基因成分，初探ddPCR技术用于检测食用植物油中转基因成分的可行性，结果显示，ddPCR检测结果与实时荧光PCR结果一致，均能检出转基因初榨菜籽油中的转基因成分，为后续开展食用植物油中转基因成分精准定量检测奠定了基础。
China is one of the biggest importers of edible vegetable oils and oilseeds. The oil made from trans-genic rapeseed has become cooking oil in consumers’ daily life if we look in view of the status of transgenic rape planting scale, thus, whether cooking oil contains transgenic ingredients concerns national regulators and the public. For a long time, detection of transgenic components in edible vegetable oil has been limited by the separation and purification of nucleic acids, and there is no effective detection standard currently. In present study, we detected exogenous transgenic components of rapeseed oil by using real-time fluorescent PCR and droplet digital PCR at the same time to see if ddPCR technology is suitable for detection of genetically modified ingredients in edible vegetable oil. The results showed that ddPCR test result is consistent with real-time fluorescence PCR, modified ingredients in virgin rapeseed oil are all detected genetically. This result laid a ground-work for accurate quantitative detection of transgenic components in edible oil in the near future.

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