Researches for natural antioxidants that play an important role in the
prevention of diseases linked to free radicals have increased in recent years. In this study,
antioxidant activity of Bauhinia rufescensLam which is a medicinal
plantwas investigated. The
methanol extract, acetone extract and water extract of leaf and trunk bark had
antioxidant activity. Three methods were used: 2,2-diphenyl-1picryl-hydrazyl
(DPPH), 2,2’-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)
(ABTS) and ferric ion reducing power (FRAP) by spectrophotometry. Phytochemical screening
was carried out followed by the quantification of the total phenolic by the
Folin-Ciocalteu method. The analysis of variance (ANOVA) with the STATISTICA
7.1 software revealed significant differences (p<0.05). Percentage inhibition (PI) and IC50 of the extracts were obtained using the OriginPro 8.5 software. These
tests show that the bark is as rich in total phenolic as the leaves. The DPPH
test shows that at 2.5 mg/ml, the bark and the leave have similar activity.
Bark has a percentage inhibition (PI) of 86.55% ± 0.026% compared to the leave
which PI is 85.63% ± 0.02%. As for the ABTS test, the bark extract reached its maximum activity
at 2.5 mg/ml with a PI of 99.81% ± 0.012% compared to the leaf extract which
has a PI of 99.
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