以酒精液态饲料评估副干酪乳杆菌Lactobacillus paracasei GKS68之护肝保健功效
Evaluation of Lactobacillus paracasei GKS68 on Hepatoprotection with Alcohol Liquid Diet

DOI: 10.12677/HJFNS.2021.101004, PP. 24-32

Keywords: 副干酪乳杆菌Lactobacillus paracasei GKS68，肝脏保健，酒精性肝病(ALD)
Lactobacillus para-casei GKS68, Hepatoprotective Effect, Alcoholic Liver Disease (ALD)

Full-Text   Cite this paper   Add to My Lib

Abstract:

过量饮酒易导致代谢过程产生过多自由基，造成氧化压力，影响脂质代谢造成脂肪累积于肝脏，长期会有慢性发炎、肝纤维化与肝癌等病变的风险。近年研究显示益生菌对人体健康扮演重要的角色。本研究以酒精液态饲料评估副干酪乳杆菌Lactobacillus paracasei GKS68之护肝保健功效。连续6周口服GKS68搭配酒精性饲料的C57BL/6雄性鼠动物模式中，小鼠之体重变化及摄取量与负对照组无显着差异；6周后牺牲进行血液生化分析，AST、ALT、TG、TC与负对照组均有显着下降。另外，肝脏相对重量、高剂量组之肝脏中TG、TC与负对照组亦有显着差异；肝脏酵素GSH含量、SOD活性在高剂量组与负对照组相比具有显着提升，显示GKS68可改善酒精引起的肝细胞损伤、有效降低酒精所造成的脂肪堆积，达到肝脏保健之功效。
Excessive drinking could increase free radicals in the metabolic process, causing oxidative stress and accumulation of abnormal metabolites in the liver which higher the risk of developing chronic disease such as inflammation, liver fibrosis, and liver cancer. Recent studies have shown that probiotics are playing an important role as beneficial supplements to human health. In this study, we evaluated the effect of probiotics Lactobacillus paracasei GKS68 (GKS68) on liver protection with alcohol-diet mouse model. Twenty C57BL/6 male mice were divided into four groups: normal diet, alcohol-diet, alcohol-diet with low-dosage of GKS68, and alcohol-diet with high-dosage of GKS68. After six weeks of treatment, there were no significant differences in body weight and food intake among the alcohol-diet group. However, the GKS68 group showed a significant decrease in serum ALT, AST, TG, TC and the relative liver weight when compared to the negative control. In addition, the levels of liver TG and TC were significantly decreased in the high-dosage of GKS68 with a comparison of alcohol-diet. A dose-dependent effect was also observed in the concentration of GSH and the SOD activity with GKS68. These results suggested that the probiotics GKS68 could reduce the liver damage caused by alcohol drinking and was a potential candidate as functional supplementary on hepatoprotection.

References

[1]	Ceni, E., Mello, T. and Galli, A. (2014) Pathogenesis of Alcoholic Liver Disease: Role of Oxidative Metabolism. World Journal of Gastroenterology, 20, 17756-17772. https://doi.org/10.3748/wjg.v20.i47.17756
[2]	Dey, A. and Cederbaum, A.I. (2006) Alcohol and Oxidative Liver Injury. Hepatology, 43, S63-S74. https://doi.org/10.1002/hep.20957
[3]	Ringseis, R., Muschick, A. and Eder, K. (2017) Dietary Oxidized Fat Pre-vents Ethanol-Induced Triacylglycerol Accumulation and Increases Expression of PPARalpha Target Genes in Rat Liver. Journal of Nutrition, 137, 77-83. https://doi.org/10.1093/jn/137.1.77
[4]	Astrup, A. (2005) The Role of Dietary Fat in Obesity. Seminars in Vascu-lar Medicine, 5, 40-47. https://doi.org/10.1055/s-2005-871740
[5]	Saadeh, S. (2007) Nonalcoholic Fatty Liver Disease and Obesity. Nu-trition in Clinical Practice, 22, 1-10. https://doi.org/10.1177/011542650702200101
[6]	Pandey, K.R., Naik, S.R. and Vakil, B.V. (2015) Probiotics, Prebiotics and Synbiotics—A Review. Journal of Food Science and Technology, 52, 7577-7587. https://doi.org/10.1007/s13197-015-1921-1
[7]	Fuller, R. (1989) Probiotics in Man and Animals. Journal of Ap-plied Bacteriology, 66, 365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
[8]	Gareau, M.G., Sherman, P.M. and Walker, W.A. (2010) Probiotics and the Gut Microbiota in Intestinal Health and Disease. Nature Reviews Gastroenterology & Hepatology, 7, 503-514. https://doi.org/10.1038/nrgastro.2010.117
[9]	Sung, V., D’Amico, F., Cabana, M.D., Chau, K., Koren, G., Savino, F., Szajewska, H., Deshpande, G., Dupont, C., Indrio, F., Mentula, S., Partty, A. and Tancredi, D. (2018) Lactobacillus reuteri to Treat Infant Colic: A Meta-Analysis. Pediatrics, 141, e20171811. https://doi.org/10.1542/peds.2017-1811
[10]	Borchers, A.T., Selmi, C., Meyers, F.J., Keen, C.L. and Gershwin, M.E. (2009) Probiotics and Immunity. The Journal of Gastroenterology, 44, 26-46. https://doi.org/10.1007/s00535-008-2296-0
[11]	Cervantes-Barragan, L., Chai, J.N., Tianero, M.D., Di Luccia, B., Ahern, P.P., Merriman, J., Cortez, V.S., Caparon, M.G., Donia, M.S., Gilfillan, S., Cella, M., Gordon, J.I., Hsieh, C.S. and Colonna, M. (2017) Lactobacillus reuteri Induces Gut Intraepithelial CD4(+)CD8alphaalpha(+) T Cells. Science, 357, 806-810. https://doi.org/10.1126/science.aah5825
[12]	Hsu, C.L., Hou, Y.H., Wang, C.S., Lin, S.W., Jhou, B.Y., Chen, C.C. and Chen, Y.L. (2019) Antiobesity and Uric Acid-Lowering Effect of Lactobacillus plantarum GKM3 in High-Fat-Diet-Induced Obese Rats. Journal of the American College of Nutrition, 38, 623-632. https://doi.org/10.1080/07315724.2019.1571454
[13]	Ji, Y., Park, S., Chung, Y., Kim, B., Park, H., Huang, E., Jeong, D., Jung, H.Y., Kim, B., Hyun, C.K. and Holzapfel, W.H. (2019) Amelioration of Obesity-Related Biomarkers by Lactobacillus sakei CJLS03 in a High-Fat Diet-Induced Obese Murine Model. Scientific Reports, 9, Article No. 6821. https://doi.org/10.1038/s41598-019-43092-y
[14]	黄彩怡. Lactobacillus paracasei TKU010所生产蛋白酶特性研究及其应用: [硕士学位论文]. 台湾: 淡江大学生命科学研究所, 2006.
[15]	Wang, W., Li, Q., Chai, W., Sun, C., Zhang, T., Zhao, C., Yuan, Y., Wang, X., Liu, H. and Ye, H. (2019) Lactobacillus paracasei Jlus66 Extenuate Oxidative Stress and Inflammation via Regulation of Intestinal Flora in Rats with Non Alcoholic Fatty Liver Disease. Food Science & Nutrition, 7, 2636-2646. https://doi.org/10.1002/fsn3.1118
[16]	Yao, F., Jia, R., Huang, H., Yu, Y., Mei, L., Bai, L., Ding, Y. and Zheng, P. (2019) Effect of Lactobacillus paracasei N1115 and Fructooligosaccharides in Nonalcoholic Fatty Liver Disease. Archives of Medical Science, 15, 1336-1344. https://doi.org/10.5114/aoms.2019.86611
[17]	Wang, C.S., Lin, S.W., Zhao, C., Chen, Y.L., Tsai, P.C. and Chen, C.C. (2018) Hypoglycemic Effect of Lactobacillus paracasei GKS6. Hans Journal of Food and Nutrition Science, 8, 9-16. https://doi.org/10.12677/HJFNS.2019.81002
[18]	Yang, L.C., Lin, S.W., Li, I.C., Chen, Y.P., Tzu, S.Y., Chou, W., Chen, C.C., Lin, W.C., Chen, Y.L. and Lin, W.H. (2020) Lactobacillus plantarum GKM3 and Lactobacillus paracasei GKS6 Supplementation Ameliorates Bone Loss in Ovariectomized Mice by Promoting Osteoblast Differentia-tion and Inhibiting Osteoclast Formation. Nutrients, 12, 1914. https://doi.org/10.3390/nu12071914
[19]	黄钰芸, 吕淑妤. 成人饮酒行为盛行率与趋势调查[C]. 休闲产业与健康促进学术研讨会. 2018: 83-87.
[20]	李亚茹, 王婧, 赵丽云, 王志宏, 于冬梅, 何宇纳, 丁钢强. 中国成年人饮酒习惯及影响因素[J]. 中华流行病学杂志, 2018, 39(7): 898-903.
[21]	WHO (2018) Global Status Report on Alcohol and Health 2018. https://www.who.int/substance_abuse/publications/global_alcohol_report/gsr_2018/en
[22]	Wheele, M.D. (2003) Endotoxin and Kupffer Cell Activation in Alcoholic Liver Disease. Alcohol Research & Health, 27, 300-306.
[23]	Venkatesan, S. and Simpson, K.J. (1989) Fatty Liver and Plasma Corticosterone Levels in Chronically Alcohol- and Pair-Fed Rats. Biochemical Society Transactions, 17, 1114-1115. https://doi.org/10.1042/bst0171114
[24]	Ceni, E., Mello, T. and Galli, A. (2014) Pathogenesis of Alcoholic Liver Disease: Role of Oxidative Metabolism. World Journal of Gastroenterology, 20, 17756-17772. https://doi.org/10.3748/wjg.v20.i47.17756
[25]	Dey, A. and Cederbaum, A.I. (2006) Alcohol and Oxidative Liver Injury. Hepatology, 43, S63-S74. https://doi.org/10.1002/hep.20957
[26]	Ringseis, R., Muschick, A. and Eder, K. (2017) Dietary Oxidized Fat Pre-vents Ethanol-Induced Triacylglycerol Accumulation and Increases Expression of PPARalpha Target Genes in Rat Liver. Journal of Nutrition, 137, 77-83. https://doi.org/10.1093/jn/137.1.77
[27]	Astrup, A. (2005) The Role of Dietary Fat in Obesity. Seminars in Vascu-lar Medicine, 5, 40-47. https://doi.org/10.1055/s-2005-871740
[28]	Saadeh, S. (2007) Nonalcoholic Fatty Liver Disease and Obesity. Nu-trition in Clinical Practice, 22, 1-10. https://doi.org/10.1177/011542650702200101
[29]	Pandey, K.R., Naik, S.R. and Vakil, B.V. (2015) Probiotics, Prebiotics and Synbiotics—A Review. Journal of Food Science and Technology, 52, 7577-7587. https://doi.org/10.1007/s13197-015-1921-1
[30]	Fuller, R. (1989) Probiotics in Man and Animals. Journal of Ap-plied Bacteriology, 66, 365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
[31]	Gareau, M.G., Sherman, P.M. and Walker, W.A. (2010) Probiotics and the Gut Microbiota in Intestinal Health and Disease. Nature Reviews Gastroenterology & Hepatology, 7, 503-514. https://doi.org/10.1038/nrgastro.2010.117
[32]	Sung, V., D’Amico, F., Cabana, M.D., Chau, K., Koren, G., Savino, F., Szajewska, H., Deshpande, G., Dupont, C., Indrio, F., Mentula, S., Partty, A. and Tancredi, D. (2018) Lactobacillus reuteri to Treat Infant Colic: A Meta-Analysis. Pediatrics, 141, e20171811. https://doi.org/10.1542/peds.2017-1811
[33]	Borchers, A.T., Selmi, C., Meyers, F.J., Keen, C.L. and Gershwin, M.E. (2009) Probiotics and Immunity. The Journal of Gastroenterology, 44, 26-46. https://doi.org/10.1007/s00535-008-2296-0
[34]	Cervantes-Barragan, L., Chai, J.N., Tianero, M.D., Di Luccia, B., Ahern, P.P., Merriman, J., Cortez, V.S., Caparon, M.G., Donia, M.S., Gilfillan, S., Cella, M., Gordon, J.I., Hsieh, C.S. and Colonna, M. (2017) Lactobacillus reuteri Induces Gut Intraepithelial CD4(+)CD8alphaalpha(+) T Cells. Science, 357, 806-810. https://doi.org/10.1126/science.aah5825
[35]	Hsu, C.L., Hou, Y.H., Wang, C.S., Lin, S.W., Jhou, B.Y., Chen, C.C. and Chen, Y.L. (2019) Antiobesity and Uric Acid-Lowering Effect of Lactobacillus plantarum GKM3 in High-Fat-Diet-Induced Obese Rats. Journal of the American College of Nutrition, 38, 623-632. https://doi.org/10.1080/07315724.2019.1571454
[36]	Ji, Y., Park, S., Chung, Y., Kim, B., Park, H., Huang, E., Jeong, D., Jung, H.Y., Kim, B., Hyun, C.K. and Holzapfel, W.H. (2019) Amelioration of Obesity-Related Biomarkers by Lactobacillus sakei CJLS03 in a High-Fat Diet-Induced Obese Murine Model. Scientific Reports, 9, Article No. 6821. https://doi.org/10.1038/s41598-019-43092-y
[37]	黄彩怡. Lactobacillus paracasei TKU010所生产蛋白酶特性研究及其应用: [硕士学位论文]. 台湾: 淡江大学生命科学研究所, 2006.
[38]	Wang, W., Li, Q., Chai, W., Sun, C., Zhang, T., Zhao, C., Yuan, Y., Wang, X., Liu, H. and Ye, H. (2019) Lactobacillus paracasei Jlus66 Extenuate Oxidative Stress and Inflammation via Regulation of Intestinal Flora in Rats with Non Alcoholic Fatty Liver Disease. Food Science & Nutrition, 7, 2636-2646. https://doi.org/10.1002/fsn3.1118
[39]	Yao, F., Jia, R., Huang, H., Yu, Y., Mei, L., Bai, L., Ding, Y. and Zheng, P. (2019) Effect of Lactobacillus paracasei N1115 and Fructooligosaccharides in Nonalcoholic Fatty Liver Disease. Archives of Medical Science, 15, 1336-1344. https://doi.org/10.5114/aoms.2019.86611
[40]	Wang, C.S., Lin, S.W., Zhao, C., Chen, Y.L., Tsai, P.C. and Chen, C.C. (2018) Hypoglycemic Effect of Lactobacillus paracasei GKS6. Hans Journal of Food and Nutrition Science, 8, 9-16. https://doi.org/10.12677/HJFNS.2019.81002
[41]	Yang, L.C., Lin, S.W., Li, I.C., Chen, Y.P., Tzu, S.Y., Chou, W., Chen, C.C., Lin, W.C., Chen, Y.L. and Lin, W.H. (2020) Lactobacillus plantarum GKM3 and Lactobacillus paracasei GKS6 Supplementation Ameliorates Bone Loss in Ovariectomized Mice by Promoting Osteoblast Differentia-tion and Inhibiting Osteoclast Formation. Nutrients, 12, 1914. https://doi.org/10.3390/nu12071914
[42]	黄钰芸, 吕淑妤. 成人饮酒行为盛行率与趋势调查[C]. 休闲产业与健康促进学术研讨会. 2018: 83-87.
[43]	李亚茹, 王婧, 赵丽云, 王志宏, 于冬梅, 何宇纳, 丁钢强. 中国成年人饮酒习惯及影响因素[J]. 中华流行病学杂志, 2018, 39(7): 898-903.
[44]	WHO (2018) Global Status Report on Alcohol and Health 2018. https://www.who.int/substance_abuse/publications/global_alcohol_report/gsr_2018/en
[45]	Wheele, M.D. (2003) Endotoxin and Kupffer Cell Activation in Alcoholic Liver Disease. Alcohol Research & Health, 27, 300-306.
[46]	Venkatesan, S. and Simpson, K.J. (1989) Fatty Liver and Plasma Corticosterone Levels in Chronically Alcohol- and Pair-Fed Rats. Biochemical Society Transactions, 17, 1114-1115. https://doi.org/10.1042/bst0171114

Full-Text