Background:Campylobacter contamination in
poultry and poultry product has been reported worldwide. The present study aims
to determine the prevalence of Campylobacter in poultry feces using selective enrichment Bolton broth and multiplex PCR. Method: Two methods were used in this study: the first wasdirect plating of poultry feces
into mCCDA agar plates. The second, three antibiotics were used at different concentrations
to add in Bolton broth supplemented. These antibiotics were Rifampicin (Oxoid, Nepean,
Ontario) with 10mg/L, colistin
(Oxoid, Nepean, Ontario) with 1mg/mL
and 2mg/mL;
trimethoprim (Oxoid, Nepean, Ontario) with 10mg/L. The colonies with typical campylobacter morphology on blood agar (little, red and ring colonies) were further identified to
the species level by multiplex polymerase chain reaction (PCR). Results: The addition of colistin (2mg/mL)
to the Bolton broth with selective supplements enhanced the selective isolation
of
References
[1]
Steinhauserova, I., Ceskova, J., Fojtikova, K., Obrovska, I. (2001) Identification of Thermophilic Campylobacter spp. by Phenotypic and Molecular Methods. Journal of Applied Microbiology, 90, 470-475. https://doi.org/10.1046/j.1365-2672.2001.01267.x
[2]
Centers for Disease Control and Prevention (2010) Campylobacter Technical Information. http://www.cdc.gov/nczved/divisions/dfbmd/diseases/campylobacter/technical.htm
[3]
Schonberg-Norio, D., Mattila, L., Lauhio, A., Katila, M.L., Kaukoranta, S.S., Koskela, M., Pajarre, S., Uksila, J., Eerola, E., Sarna, S. and Rautelin, H. (2010) Patient-Reported Complications Associated with Campylobacter jejuni Infection. Epidemiology & Infection, 138, 1004-1011. https://doi.org/10.1017/S0950268809991099
[4]
Taremi, M., Mehdi Soltan Dallal, M., Gachkar, L., MoezArdalan, S., Zolfagharian, K. and Reza Zali, M. (2006) Prevalence and Antimicrobial Resistance of Campylobacter Isolated from Retail Raw Chicken and Beef Meat, Tehran, Iran. International Journal of Food Microbiology, 108, 401-403. https://doi.org/10.1016/j.ijfoodmicro.2005.12.010
[5]
Prachantasena, S., Charununtakorn, P., Muangnoicharoen, S., Hankla, L., Techawal, N., Chaveerach, P., Tuitemwong, P., Chokesajjawatee, N., Williams, N., Humphrey, T. and Luangtongkum, T. (2016) Distribution and Genetic Profiles of Campylobacter in Commercial Broiler Production from Breeder to Slaughter in Thailand. PLoS ONE, 11, e0149585. https://doi.org/10.1371/journal.pone.0149585
[6]
Reddy, S. and Zishiri, O.T. (2017) Detection and Prevalence of Antimicrobial Resistance Genes in Campylobacter spp. Isolated from Chickens and Humans. Onderstepoort Journal of Veterinary Research, 84, Article No. a1411. https://doi.org/10.4102/ojvr.v84i1.1411
[7]
Yamazaki-Matsune, W., Taguchi, M., Seto, K., Kawahara, R., Kawatsu, K., Kumeda, Y., Kitazato, M., Nukina, M., Misawa, N. and Tsukamoto, T. (2007) Development of a Multiplex PCR Assay for Identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. Journal of Medical Microbiology, 56, 1467-1473. https://doi.org/10.1099/jmm.0.47363-0
[8]
Rawat, N., Maansi, Kumar, D. and Upadhyay, A.K. (2018) Virulence Typing and Antibiotic Susceptibility Profiling of Thermophilic Campylobacters Isolated from Poultry, Animal, and Human Species. Veterinary World, 11, 1698-1705. https://doi.org/10.14202/vetworld.2018.1698-1705
[9]
Bonkoungou, I.J.O., Haukka, K., Österblad, M., Hakanen, A.J., Traoré, A.S., Barro, N. and Siitonen, A. (2013) Bacterial and Viral Aetiology of Childhood Diarrhea in Ouagadougou, Burkina Faso. BMC Pediatrics, 13, Article No. 36. https://doi.org/10.1186/1471-2431-13-36
[10]
Kagambèga, A., Thibodeau, A., Trinetta, V., Soro, K.D., Sama, N.F., Bako, E., Bouda, S.C., Wereme, A., Fravalo, P. and Barro, N. (2018) Salmonella spp. and Campylobacter spp. in Poultry Feces and Carcasses in Ouagadougou, Burkina Faso. Food Science & Nutrition, 6, 1601-1606. https://doi.org/10.1002/fsn3.725
[11]
Kim, J., Oh, E., Banting, G.S., Braithwaite, S., Chui, L., Ashbolt, N.J., Neumann, N.F. and Jeon, B. (2016) An Improved Culture Method for Selective Isolation of Campylobacter jejuni from Wastewater. Frontiers in Microbiology, 7, 1345. https://doi.org/10.3389/fmicb.2016.01345
[12]
Yoo, J.H., Choi, N.Y., Bae, Y.M., Lee, J.S. and Lee, S.Y. (2014) Development of a Selective Agar Plate for the Detection of Campylobacter spp. in Fresh Produce. International Journal of Food Microbiology, 189, 67-74. https://doi.org/10.1016/j.ijfoodmicro.2014.07.032
[13]
Chon, J.W., Kim, H., Yim, J.H., Park, J.H., Kim, M.S. and Seo, K.H. (2013) Development of a Selective Enrichment Broth Supplemented with Bacteriological Charcoal and a High Concentration of Polymyxin B for the Detection of Campylobacter jejuni and Campylobacter coli in Chicken Carcass Rinses. International Journal of Food Microbiology, 162, 308-310. https://doi.org/10.1016/j.ijfoodmicro.2013.01.018
[14]
Colles, F.M., McCarthy, N.D., Bliss, C.M., Layton, R. and Maiden, M.C. (2015) The Long-Term Dynamics of Campylobacter Colonizing a Free-Range Broiler Breeder Flock: An Observational Study. Environmental Microbiology, 17, 938-946. https://doi.org/10.1111/1462-2920.12415
[15]
El-Shibiny, A., Connerton, P.L. and Connerton, I.F. (2007) Campylobacter Succession in Broiler Chickens. Veterinary Microbiology, 125, 323-332. https://doi.org/10.1016/j.vetmic.2007.05.023
[16]
Osbjer, K., Boqvist, S., Sokerya, S., Cheng, K., San, S., Davun, H., Rautelin, H. and Magnusson, U. (2016) Risk Factors Associated with Campylobacter Detected by PCR in Humans and Animals in Rural Cambodia. Epidemiology & Infection, 144, 2979-2988. https://doi.org/10.1017/S095026881600114X
[17]
Kaakoush, N.O., Castano-Rodriguez, N., Mitchell, H.M. and Man, S.I.M. (2015) Global Epidemiology of Campylobacter Infection. Clinical Microbiology Reviews, 28, 687-720. https://doi.org/10.1128/CMR.00006-15
[18]
Whiley, H., Van Den Akker, B., Giglio, S., and Bentham, R. (2013) The Role of Environmental Reservoirs in Human Campylobacteriosis. International Journal of Environmental Research and Public Health, 10, 5886-5907. https://doi.org/10.3390/ijerph10115886
