全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
-  2014 

ATPase-Independent Type-III Protein Secretion in Salmonella enterica

DOI: 10.1371/journal.pgen.1004800

Keywords: Adenosine triphosphatase,Secretion systems,Protein secretion,Secretion,Mutant strains,Flagella,Pathogen motility,Deletion mutagenesis

Full-Text   Cite this paper   Add to My Lib

Abstract:

Type-III protein secretion systems are utilized by gram-negative pathogens to secrete building blocks of the bacterial flagellum, virulence effectors from the cytoplasm into host cells, and structural subunits of the needle complex. The flagellar type-III secretion apparatus utilizes both the energy of the proton motive force and ATP hydrolysis to energize substrate unfolding and translocation. We report formation of functional flagella in the absence of type-III ATPase activity by mutations that increased the proton motive force and flagellar substrate levels. We additionally show that increased proton motive force bypassed the requirement of the Salmonella pathogenicity island 1 virulence-associated type-III ATPase for secretion. Our data support a role for type-III ATPases in enhancing secretion efficiency under limited secretion substrate concentrations and reveal the dispensability of ATPase activity in the type-III protein export process

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133