神经酸是一种含有24个碳原子的长链单不饱和脂肪酸，是迄今为止世界上发现的能促进受损神经细胞组织修复和再生的特效物质，其在蒜头果的种子中含量丰富而广为国内外关注。本文以蒜头果(Malania oleifera)种子为研究材料，以石油醚为溶剂索氏提取法获得种子油，以结晶次数、萃取料液比和萃取次数为因素，采用正交试验设计法，以HPLC法测定的神经酸含量为评价指标，对蒜头果种子油中的神经酸的纯化方法进行优化，最优条件为结晶次数为2次，萃取料液比为1:100，萃取次数为1次。最优条件下所得产物的神经酸的含量为70.19%。该方法稳定性好，操作简单，为从蒜头果种子油中制备神经酸产品的产业化生产提供参考数据。
Nervonic acid, a long-chain monounsaturated fatty acid with 24 carbon atoms, is a special substance found in the world that can promote the repair and regeneration of damaged nerve cells. It is abundant in the seed of Malania oleifera, and has been attracted much attention at home and abroad. In this paper, the seeds of M. oleifera are used as the research materials, and the seed oil is obtained by the Soxhlet extraction method using petroleumether as the solvent. The number of crystallization, the ratio of extraction material to liquid and the number of extraction are factors. Using the orthogonal experiment design method, the HPLC method for determination of nervonic acid content as evaluation index, optimization of the purification method of nervonic acid in seeds oil of M. oleifera, the optimal condition is that the crystallization times are 2 times, the extraction material-liquid ratio is 1:100, and the extraction time is 1 time. Under the optimal conditions, the content of nervonic acid was 70.19%. This method has good stability and simple operation, and provides reference data for the industrial production of nervonic acid from the seed oil of M. oleifera.
