Molecular Investigation on Clinopathological, Genetic and Biochemical Changes in Channa punctata Infected with Internal Parasites and Subjected to Metals Pollution in Chittagong, Bangladesh

This study was carried out to investigate the effects of parasites and metals in the fishes of fresh water sources of three different stations of Hathazari upazilla, Chittagong, Bangladesh, by analyzing blood biochemical parameters and body composition of Channa punctata. The results indicate that, the highest rate of parasitic infection changes the blood parameters spontaneously. Findings of blood biochemical factors (Blood sugar, Hemoglobin and Creatinine) revealed significant variation between infected and uninfected fishes. Considerable works mainly on systematics, nature of infestation and pathology of different groups of fish parasites - Cystode, Trematodes and Nematodes have been done. Different species of parasites as endoparasitic Cystode and monogenetic Trematodes are recorded mainly from fish species of different water sources. One helminth parasite Nematode is also reported from those fishes. Much attention has been given on observing intestine, liver and kidney. Molecular observation including DNA extraction has been done from both infected and healthy fish blood sample. Purification of DNA has been confirmed by Nano drop. Recommendation has been made for the future works on parasitology for sustainable production of healthy fishes by heavy metal detection, molecular observation and as well as mutation detection.