TAK-242对人多发性骨髓瘤细胞增殖及凋亡的影响
DOI: 10.3971/j.issn.1000-8578.2019.18.1743
Keywords: 多发性骨髓瘤,TAK-242,增殖,凋亡,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Current Status and Challenges in Treatment of Relapsed/Refractory Multiple Myeloma,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Incidence of Multiple Myeloma in Shenyang from 2009 to 2014,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Downregulation of Skp2 Expression Inhibits Malignant Phenotype of Osteosarcoma U2OS Cells
Abstract:
摘要 目的 探讨Toll样受体4（TLR4）特异性抑制剂TAK-242对人多发性骨髓瘤细胞株RPMI8226增殖及凋亡的影响。方法 取对数期生长的人多发性骨髓瘤细胞株RPMI8226分为A、B、C组及对照组（Control），A、B、C组分别加入终浓度为20、40、80 μmol/L的TAK-242，对照组不加抑制剂，培养24 h后，采用CCK-8法检测细胞增殖抑制率；Annexin-V/PI检测细胞凋亡率；RT-PCR检测细胞TLR4、Myd88 mRNA的表达水平；Western blot检测细胞Myd88、NF-κB的蛋白表达情况。结果 A、B、C组细胞增殖抑制率、凋亡率升高，TLR4、Myd88 mRNA表达水平及Myd88、NF-κB的蛋白表达水平降低，各浓度组之间的差异均有统计学意义（P<0.05）。结论 TAK-242可抑制人多发性骨髓瘤细胞RPMI8226的增殖，促进细胞凋亡，其机制可能为NF-κB信号通路功能被抑制
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