Human adipose tissue protein analyses using capillary western blot technology

Detection of CD36 (a), total AKT, pAKTS473 (b) and total Erk1/2, pErk1/2 (c) in Capillary western blot (Wes？) assay, using two-fold dilutions of calibrator samples. The calibrator sample was obtained from a pooled whole-tissue extract from 3 to 4 surgical waste adipose tissues. Capillary western blot results were shown as gel-like image view in left panel and electropherograms in right panel, showing decreasing intensities of bands and decreasing peak areas with serial dilutions of loading sample