Notch signaling triggered via the ligand DLL4 impedes M2 macrophage differentiation and promotes their apoptosis

DLL4 impairs M2 macrophage polarization. a Phenotype analysis of macrophages before (M0) and after polarization in M1 or M2 macrophages for 24 h with LPS and IFN-γ or IL-4, respectively (n？=？5). b Phenotype analysis of M0, M1 and M2 macrophages polarized during 24 h with (+rhDLL4) or without (？rhDLL4) coated rhDLL4 (n？=？5). Data are expressed as fold increases ± SD from 5 independant experiments. M1-specific and M2-specific makers are indicated. Mann-Whitney test was used for statistical analysis of the results (*p？<？0.05 and ** if p？<？0.01