Interfering cellular lactate homeostasis overcomes Taxol resistance of breast cancer cells through the microRNA-124-mediated lactate transporter (MCT1) inhibition

Lactate homeostasis is positively correlated with Taxol resistance of breast cancer cells. a BT474, SKBR3 and MCf7 cells were treated with indicated concentrations for 48 h, the protein levels and (b–d) mRNA levels of LHDA and MCT1 were detected by Western blot and qRT-PCR, respectively. e–g Lactate productions of BT474, SKBR3 and MCF7 cells were measured under indicated Taxol treatments. h Cell viability assays were performed in MCF7 parental and Taxol resistant cells under 0, 5, 10, 20, 40, 80, 160 and 320 nM Taxol treatments for 48 h. i Protein and (j, k) mRNA expressions of MCT1 and LDHA were detected in MCF7 parental and Taxol resistant cells by Western blot and qRT-PCR, respectively. β-actin is a loading control. l Glucose consumption and (M) lactate production were measured in MCF7 parental and Taxol resistant cells. *p？<？0.05; **p？<？0.01; ***p？<？0.00