顺铂诱导人鼻咽癌CNE2细胞衰老及其相关基因表达谱的研究
DOI: 10.3971/j.issn.1000-8578.2015.01.002
Keywords: 顺铂,肿瘤细胞早衰,鼻咽癌,细胞周期阻滞,Clinical Analysis of Radiotherapy Combined with Cisplatin-based Chemotherapy for Locally Advanced Esophageal Squamous Cell Carcinoma,Comparison of Long-term Efficacy of IMRT plus Concurrent Chemotherapy Versus Neoadjuvant Chemotherapy Followed by IMRT plus Concurrent Chemotherapy for Locoregionally Advanced Nasopharyngeal Carcinoma,Correlation Between Skull Base Invasion and Bone Metastases in Locally Advanced Nasopharyngeal Carcinoma Patients,Construction of RNAi Targeting CLU Gene and Effect of CLU on Biological Function of Nasopharyngeal Cancer Cells,GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP,Establishment of DDP Resistant Variant of Triple Negative Breast Cancer Cell Line MDA-MB-231/DDP and Its Appraisal,Killing Effect of Paclitaxel and Cisplatin on EGFR-wild and Mutant Lung Adenocarcinoma Cells and Related Molecular Mechanism,Research Progress of Neoadjuvant Chemotherapy for Locally Advanced Nasopharyngeal Carcinoma,Effect of 5-Azacytidine on hTERT mRNA Expression and Telomerase Activity in Human Nasopharyngeal Carcinoma Cells,Expression of Annexin A2 in Nasopharyngeal Carcinoma Tissues and Its Clinical Significance,Trends on Morbidity of Nasopharyngeal Carcinoma Among Residents in Pudong New Area of Shanghai, 2002-2013,Role of p53β Isoform in Inhibitory Effect of rmhTNF Combined with Cisplatin on Human Gastric Cancer Cell Growth,Effects of High Mobility Group Box-1 Protein on Migration and Invasion Ability of Nasopharyngeal Carcinoma Cell Line C666-1 in vitro,Antiemetic Effect of Aprepitant for Cisplatin-induced Nausea and Vomiting in Melanoma Patients,Correlation of DNA Repair Gene XRCC1 Single Nucleotide Polymorphism with Sensitivity of Nasopharyngeal Carcinoma Patients to Platinum Chemotherapy
Abstract:
摘要 目的 探讨顺铂体外诱导人鼻咽癌CNE2细胞衰老的效应及其分子机制。方法 采用 MTT 比色法观察顺铂对CNE2细胞增殖的抑制作用，衰老相关-β-半乳糖苷酶（SA-β-gal）活性评判细胞衰老，流式细胞术分析细胞周期分布，实时定量PCR法检测78个人类细胞衰老相关基因的表达。结 果 顺铂对CNE2细胞的增殖具有明显抑制作用；1.0 mg/L顺铂处理后第6和第8天检测SA-β-gal染色阳性细胞数分别达到67.63%和89.22%，细胞阻滞于G2 期；TP53、TP63、p16Ink4a，p27Kip1、PTEN、RB1、Cdc25C、TBX3、Gadd45a、IGF1R、PIK3CA、BMI-1、B2M、MORC3、MYC和SPARC等16个衰老相关基因的mRNA水平显著升高（P＜0.05或0.01），Cyclin A2、Cyclin B1、Cyclin E1、CDK6、ATM、ETS2、MDM2、E2F1、ETS1、FN1、IGFBP3、RBL2、SERPINB2及SIRT1等14个基因表达水平下降（P＜0.05 或 0.01）。结论 顺铂能够在体外诱导CNE2细胞衰老，其机制涉及p16和p27介导的p53-pRB和PTEN衰老信号调控网络
Full-Text
