免疫组织化学与聚合酶链反应在结肠癌微卫星状态筛选中的比较
DOI: 10.3971/j.issn.1000-8578.2015.12.013
Keywords: 免疫组织化学,聚合酶链反应,结肠癌,微卫星不稳定,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Jinlong Capsule on Vasculogenic Mimicry and Mig-7 of Colon Cancer,Expression and Correlation of Id-1, MMP-9 and LMVD in Colorectal Adenocarcinoma Tissues,Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism,One Case Report of Ablation by Nano Knife on Unresectable Liver Metastases from Colon Cancer,Prognostic Significance of COX-2, NF-κB, WT-1 and PTEN Expression in Malignant Peritoneal Mesothelioma,Recent Advances of Correlation of Mismatch Repair System hMutS, hMutL Variation and Colorectal Cancer,Expressions of MACC1 and c-Met Proteins in Lung Adenocarcinoma and Their Correlation with Postoperative Recurrence,Expression and Clinical Significance of Artemin Protein in Triple-negative and Nontriple-negative Breast Cancer Tissues,Role of miR-138 in Invasion of Human Colon Cancer Cells SW620 by Regulating PDK1 Expression,Effects of Different Doses of Bevacizumab on Irinotecan Distribution in Nude Mice Bearing Human Colon Cancer Cell Xenografts,Clinical Significance of Immunohistochemistry in Detecting Epidermal Growth Factor Receptor Mutation in Lung Cancer,Clinical Significance of Different Human mcl1 Subtypes Expression in Colorectal Cancer Tissues,Expression of Annexin A2 in Nasopharyngeal Carcinoma Tissues and Its Clinical Significance,Transcriptional Levels of Mitochondrial DNA ATPase6 and ATPase8 in Breast Invasive Ductal Carcinoma and Related Significance
Abstract:
摘要 目的 比较免疫组织化学（IHC）与聚合酶链反应（PCR）检测结肠癌微卫星不稳定状态的一致性，评价IHC检测微卫星不稳定状态的可行性。方法 采用IHC和PCR两种方法分别检测80例结肠癌中微卫星不稳定状态。结果 （1）IHC法检测结果显示癌旁正常黏膜中错配修复蛋白（包括MLH1、PMS2、MSH2、MSH6）均阳性表达。四个蛋白表达缺失率分别为23.8%（19/80）、21.2%（17/80）、7.5%（6/80）、3.7%（3/80），其中有16例显示MLH1、PMS2表达同时缺失，3例显示MSH2和MSH6表达同时缺失。19例（23.8%）显示为高频微卫星不稳定，剩余病例显示微卫星稳定状态或低频微卫星不稳定；（2）PCR产物测序结果显示18例为高频微卫星不稳定，5例为低频微卫星不稳定，其余病例均为微卫星稳定状态；（3）两种方法具有高度一致性。结论 与PCR法相比，IHC具有操作简单、耗时短、费用低以及对实验仪器条件要求不高等优点，具有较高的临床应用价值，可以作为检测结肠癌微卫星不稳定状态的首选方法
Full-Text
