食管癌细胞线粒体中MYH9蛋白的分布及与SLP-2结合的研究
DOI: 10.3971/j.issn.1000-8578.2015.03.004
Keywords: 非肌性肌球蛋白重链9,SLP-2,线粒体,食管鳞癌细胞,Clinical Analysis of Radiotherapy Combined with Cisplatin-based Chemotherapy for Locally Advanced Esophageal Squamous Cell Carcinoma,Relationship Between NF-κB and Epithelial-to-mesenchymal Transition of Radiationinduced Human Esophageal Carcinoma Cells,Effect of Gene Silencing of RNF2 on Radiosensitivity of Esophageal Carcinoma Cells,Expression and Clinical Significance of Stem Cells Gene Lgr5 in Esophageal Squamous Cell Carcinoma Tissues,Department of Thoracic Surgery, Zhejiang Cancer Hospital, Hangzhou 310022, China,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway,Epidemiologic Investigation on Mortality Due to Esophagus Cancer in Residents in Xiamen, 2005-2014,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Prognosis Factors for Patients with Stage pT3N0M0 Thoracic Esophageal Squamous Cell Carcinoma after Radical Resection,Expression of EZH2 and H3K27me3 in Esophageal Squamous Cell Carcinoma and Their Significance,Expression of DMRT2 in Esophageal Squamous Cell Carcinoma Tissues and Related Clinical Significance,Relationship of Secreted Protein Acidic and Rich in Cysteine Protein Expression with Prognosis of Postoperative Patients with Esophageal Squamous Cell Carcinoma,Metastasis?associated Lung Adenocarcinoma Transcript1(MALAT1) Expression in Middle Thoracic Esophageal Squamous Cell Cancer Tissues and Its Relationship with Prognosis,Relationship Between Lymphocyte Nadir and Radiotherapy Efficacy or Prognosis of Patients with Esophageal Carcinoma,Support Vector Machine Predicts Survival of Esophageal Squamous Cell Carcinoma Patients
Abstract:
摘要 目的 研究非肌性肌球蛋白重链9（myosin, heavy chain 9, non-muscle, MYH9）蛋白在食管鳞癌细胞线粒体内的分布，及MYH9蛋白与线粒体相关蛋白SLP-2的结合情况。方法 使用抗stomatinlikeprotein 2 （SLP-2）抗体，在食管鳞癌细胞系KYSE150细胞的裂解液里进行免疫共沉淀，共沉淀物使用MALDI-TOF-MS蛋白质质谱进行分析，以分析与线粒体相关蛋白SLP-2结合的分子。对分析结果再继续使用免疫共沉淀及GST pull down法进行验证。使用蔗糖密度梯度离心纯化分离细胞线粒体，利用Western blot明确MYH9在食管癌细胞线粒体内的分布。结果 在食管鳞癌细胞系KYSE150细胞的裂解液内，MYH9蛋白可与SLP-2蛋白共沉淀，并且MYH9蛋白也能与GST-SLP-2结合。MYH9蛋白既存在于KYSE150细胞分离纯化后的细胞质中，也存在于分离出的线粒体中。结论 MYH9在食管癌细胞内部分分布于线粒体中，并与线粒体相关蛋白SLP-2相互结合。MYH9可能影响食管癌细胞线粒体的功能
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