灵芝多糖对T24荷瘤裸鼠化疗效果及其免疫逃逸的影响
DOI: 10.3971/j.issn.1000-8578.2015.05.008
Keywords: A Case Report,GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP,Establishment of DDP Resistant Variant of Triple Negative Breast Cancer Cell Line MDA-MB-231/DDP and Its Appraisal,Killing Effect of Paclitaxel and Cisplatin on EGFR-wild and Mutant Lung Adenocarcinoma Cells and Related Molecular Mechanism,Role of p53β Isoform in Inhibitory Effect of rmhTNF Combined with Cisplatin on Human Gastric Cancer Cell Growth,Antiemetic Effect of Aprepitant for Cisplatin-induced Nausea and Vomiting in Melanoma Patients,Comparison of Acute Toxicity between Intensity-modulated Radiotherapy with Cisplatin Alone Concurrent Chemotherapy Weekly and Every Three Weeks for Nasopharyngeal Carcinoma Patients,Protective Effect of Grape Seed Proanthocyanidin Extract on Cisplatin-induced Damage in Mice Testes Sertoli Cells TM4,Effect of Endoplasmic Reticulum Status on Sensitivity of Ovarian Cancer Cells to Cisplatin,Short-term Effect of Endostar/Bevacizumab in Combination with Paclitaxel and Platinum for Advanced Lung Adenocarcinoma,Cisplatin-induced Senescence and Senescence-related Gene Expression Profiles in#br# Human Nasopharyngeal Carcinoma Cells CNE2,Effect of Interfering Tumor Microenvironment on Homing and Killing Activity of Cytotoxic T Lymphocytes,Docetaxel, Cisplatin and 5-fluorouracil (DCF) Compared with Epirubicin, Cisplatin and#br# 5-fluorouracil (ECF) Regimens for Advanced Gastric Cancer: A Meta-analysis,Inhibition of Cilengitide on Tumor Growth of A Nude Mouse Model with Lung#br# Adenocarcinoma Cell Line A549 and Its Mechanism
Abstract:
摘要 目的 探讨灵芝多糖（ganoderma lucidum polysaccharide, GLP）对膀胱癌化疗效果的影响及 其机制。 方法 建立T24荷瘤裸鼠模型，分组给药后测定脾脏指数，利用流式细胞仪检测小鼠脾脏和 血液中相关淋巴细胞亚群所占比例，采用双抗体夹心（ELISA）法检测荷瘤小鼠血清IL-10、VEGF、 TGF-β、IL-2和TNF-α含量，应用反转录-聚合酶链反应（RT-PCR）和Western blot检测小鼠脾脏IL-2 和TNF-α的表达。 结果 在荷瘤裸鼠模型中，灵芝多糖（200 mg/kg）灌胃18 d后可显著增加脾脏指 数（P<0.001）；顺铂+灵芝多糖组中小鼠脾脏及血液中的淋巴细胞（CD4、CD8、NK及DC）都较 顺铂组提高，而髓源性抑制细胞(myeloid derived suppressor cell, MDSC）含量降低。通过ELISA检测 发现灵芝多糖能明显增加外周血液中相关免疫因子（IL-10、VEGF、TGF-β、IL-2和TNF-α）的含量 （P<0.05）。 RT-PCR及Western blot的结果也证实了在灵芝多糖可增强小鼠化疗后脾脏中IL-2和TNF-α 的表达。结论 灵芝多糖能通过提升脾指数，增加脾脏和外周血相关淋巴细胞及免疫因子的含量，并 通过增强血清中IL-2和TNF-α的表达来提高小鼠的免疫力，增强抗肿瘤作用，其机制可能与灵芝多糖 降低免疫逃逸功能相关
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