跨膜接头蛋白PAG1过表达对皮肤鳞癌细胞A431运动能力的影响
DOI: 10.3971/j.issn.1000-8578.2016.09.002
Keywords: 皮肤鳞癌,PAG1,迁移,侵袭,NDRG2 Inhibits Migration of Breast Cancer Cells Through Upregulating PTEN Expression,Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway,Effect of Jinlong Capsule on Vasculogenic Mimicry and Mig-7 of Colon Cancer,siRNA-mediated Inhibition of MTH1 Gene Expression Promotes Apoptosis and Reduces Migration of Hepatocellular Carcinoma HepG2 Cells,Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism,Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway,Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism,Progress of TBX3 in Development of Malignant Tumors,Expressions of MMP-7 mRNA, sMICA, VEGF in Peripheral Blood of Lung Cancer Patients and Their Relationships with Invasion and Metastasis,Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells
Abstract:
摘要 目的 研究跨膜接头蛋白PAG1过表达对人皮肤鳞状细胞癌A431运动能力的影响。方法 构建PAG1-EGFP融合蛋白的慢病毒表达载体，采用反转录病毒转染的方法建立PAG1过表达的A431细胞株，实验分为3组：亲本细胞组（未进行基因转染的A431细胞）、对照组（A431细胞转染仅含EGFP阴性对照病毒）、实验组（A431细胞转染PAG1-EGFP病毒）。流式细胞术检测细胞转染率，实时荧光定量PCR及Western blot检测转染后PAG1 mRNA及其蛋白表达，进一步验证细胞转染成功与否；利用划痕修复实验、Transwell迁移实验、侵袭实验三种不同的方法检测PAG1基因的过表达是否会对皮肤鳞癌细胞的生长、迁移和侵袭能力产生影响。 结果 实验组和对照组目的基因病毒的转染表达率分别为（94.97±0.15）%、（94.60±0.35）%；实验组细胞中PAG1基因mRNA的表达量约为亲本细胞组的1.6倍（P=0.000），转染后实验组中PAG1蛋白的相对表达水平明显增加（P=0.000），建立了稳定过表达PAG1的A431细胞系；PAG1过表达明显降低A431细胞愈合率（P=0.000）；实验组A431细胞迁移、侵袭能力明显降低（P=0.001, P=0.000）。结论 PAG1的过表达能抑制人皮肤鳞癌细胞运动能力，影响肿瘤的浸润及远处转移
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