The sweet taste of Stevia leaves makes it a
potential substitute for table sugar which can be used to sweeten foods and
beverages. However, the limited planting materials become a constrained to
large production; hence
the experiment aims to investigate the different part and methods of
propagation for stevia specifically use of different rooting hormones. The experiment was laid out in 3 × 4 factorial
arranged in
Randomized Complete Block Design. It consists of 3 types of cutting (shoot tips, intermediate
stem and basal stem part) and four kinds of commercial hormones (miracle gro, rootech gel,
NAA and control). Results showed that the highest percentage survival of stevia
was obtained from shoot tips (93.92%) which differed statistically from those
intermediate (91.00%) and basal stem cuttings (85.51%). On the other hand,
basal stem cutting significantly has the lowest percent survival. Results
revealed that shoot tip cuttings treated with Rootech Gel developed roots early
(6.92 days), with most number of roots (13.70), longer roots (3.33 cm), and
96.38% survival.
References
[1]
Rayaguru, K. and Khan, M.K. (2008) Post-Harvest Management of Stevia Leaves: A Review. Journal of Food Science and Technology, 45, 391-397.
[2]
Kinghorn, A.D. (1987) Biologically Active Compounds from Plants with Reputed Medicinal and Sweetening Properties. Journal National Production, 50, 1009-1024.
https://doi.org/10.1021/np50054a002
[3]
Jitendra, M., Monika, S., Ratan, S.D., Priyanka, G., Priyanka, S. and Kiran, D.J. (2012) Micropropagation of an Anti-Diabetic Plant—Stevia rebaudiana Bertoni, Natural Sweetener in Hadoti Region of South-East Rajasthan, India. ISCA Journal of Biological Sciences, 1, 37-42.
[4]
Castillo, R.R. (2018) The Philippines Is a Diabetes ‘Hotspot’. Philippine Daily Inquirer.
[5]
PhilStar (2017) Potential of Stevia in the Philippines.
[6]
Marc, S. (2008) Concern Grows Over Increase in Diabetes Around World. The New York Times Washington, World Population Increasing over Diabetics.
[7]
Miracle-Gro Lawn Product, Inc. (2014) Scotts Miracle-Gro Company. American Multinational Corporation, Marysville, OH.
Adams, C.R., Bamford, K.M. and Early, M.P. (2008) Principles of Horticulture. 5th Edition, Butterworth-Heinemann, Oxford, 149-165.
[11]
Kassahun, B.M. and Mekonnen, S.A. (2012) Effect of Cutting Position and Rooting. The African Journal of Plant Science and Biotechnology, 6, 5-8.
[12]
Abdullateef, R.A. (2012) Effects of Stem Cutting Types, Position and Hormonal Factors on Rooting in Stevia rebaudiana Bertoni. Journal of Agricultural Science, 4, 49-57. https://doi.org/10.5539/jas.v4n1p49
[13]
Ingle, M.R. and Venugopal, C.K. (2009) Effect of Different Growth Regulators on Rooting of Stevia (Stevia rebaudiana Bertoni) Cuttings. Karnataka Journal of Agricultural Sciences, 22,455-456.
[14]
Rout, G.R. (2006) Effect of Auxins on Adventitious Root Development from Single Node Cuttings of Camellia sinensis (L.) Kuntze and Associated Biochemical Changes. Plant Growth Regulation, 48, 111–117.
https://doi.org/10.1007/s10725-005-5665-1
[15]
Goyal, P., Kachhwaha, S. and Kothari, S.L. (2012) Micropropagation of Pithecellobium dulce (Roxb.) Benth—A Multipurpose Leguminous Tree and Assessment of Genetic Fidelity of Micropropagated Plants Using Molecular Markers. Physiology and Molecular Biology of Plants, 18, 169-176.
https://doi.org/10.1007/s12298-012-0112-z
[16]
Zolman, B.K., Martinez, N., Millius, A., Adham, A.R. and Bartel, B. (2008) Identification and Characterization of Arabidopsis Indole-3-Butyric Acid Response Mutants Defective in Novel Peroxisomal Enzymes. Genetics, 180, 237-251.
https://doi.org/10.1534/genetics.108.090399
[17]
Ludwig-Müller, J. (2000) Indole-3-Butyric Acid in Plant Growth and Development. Plant Growth Regulation, 32, 219-230. https://doi.org/10.1023/A:1010746806891
[18]
Ludwig-Müller, J., Hilgenberg, W. and Epstein, E. (1995) The in Vitro Biosynthesis of Indole-3-Butyric Acid in Maize. Phytochemistry, 40, 61-68.
https://doi.org/10.1016/0031-9422(95)00259-A
[19]
Ludwig-Müller, J. (2007) Indole-3-Butyric Acid Synthesis in Ecotypes and Mutants of Arabidopsis Thaliana under Different Growth Conditions. Journal of Plant Physiology, 164, 47–59. https://doi.org/10.1016/j.jplph.2005.10.008
[20]
Elisabeta, S., Susaj, L. and Kallco, I. (2014) Effect of Different NAA and IBA Concentrations on Rooting of Vegetative Cuttings of Two Rose Cultivars. Research Journal of Agricultural Science, 44, 121-127.
[21]
Pigatto, G.B., Nunes, E., Gomes, J., De Cássia, T., Ferriani, A.P. and Deschamps, C. (2017) Effects of Indolebutyric Acid, Stem Cutting Positions and Substrates on the Vegetative Propagation of Stevia rebaudiana Bertoni. Revista Colombiana De Ciencias Hortícolas, 12, 202-211. https://doi.org/10.17584/rcch.2018v12i1.6631
