Sugarcane burning during harvest and non-harvest season emits various pollutants like volatile organic compounds (VOCs), alkanes, and PAHs (Polyaromatic hydrocarbons) in the surrounding environment. Among these pollutants, PAHs are of uttermost concern due to their high level of toxicity. Burning of sugarcane bagase in sugar mill results in the production of fly ash. Fly ash is produced as a result of sugarcane bagasse burning in sugar mills. In present study, fly ash that comes out from the sugar mill chimney was collected from Western Uttar Pradesh, India and used for further analysis. High temperature and incomplete combustion inside chimney lead to the formation of PAHs. Extraction of PAHs present in fly ash samples was done by ultrasonication method and was identified with GC-FID (gas chromatography-flame ionization detector). Results exhibit the presence of eight PAHs in fly ash samples where the Benzo(a)pyrene and Naphthalene were found to be in high concentration. Furthermore, we have evaluated toxic effects of fly ash and Polyaromatic hydrocarbons (Standard of BaP & Nap) through different methods i.e. MTT, ROS and comet assay. Significant reduction (p < 0.001) in cell viability was noted in cells treated with fly ash as compared to control. Fly ash samples were also found to induce significant oxidative stress in HeLa cells, which ultimately causes DNA damage. Therefore, it may be concluded that the fly ash samples are toxic to the environment due to the presence of PAHs. Hence, the present study plays an important role in determining the harmful effects of PAHs and their source of occurrence.
References
[1]
Marotrao, K.P. (2017) Effect of Drip Irrigation on Sugarcane Production: A Case Study. International Journal of Tropical Agriculture, 35, 303-313.
Srinivasan, R. and Sathiya, K. (2010) Experimental Study on Bagasse Ash in Concrete. International Journal for Service Learning in Engineering, Humanitarian Engineering and Social Entrepreneurship, 5, 60-66. https://doi.org/10.24908/ijsle.v5i2.2992
[4]
Mugica-Alvarez, V., Santiago-de la Rosa, N., Figueroa-Lara, J., Flores-Rodríguez, J., Torres-Rodríguez, M. and Magaña-Reyes, M. (2015) Emissions of PAHs Derived from Sugarcane Burning and Processing in Chiapas and Morelos México. Science of the Total Environment, 527, 474-482. https://doi.org/10.1016/j.scitotenv.2015.04.089
[5]
Choi, H., Harrison, R., Komulainen, H. and Delgado, J. (2010) Polycyclic Aromatic Hydrocarbons. WHO Guidelines for Indoor Air Quality: Selected Pollutants. World Health Organization, Geneva, 1-12.
[6]
Rengarajan, T., Rajendran, P., Nandakumar, N., Lokeshkumar, B., Rajendran, P. and Nishigaki, I. (2015) Exposure to Polycyclic Aromatic Hydrocarbons with Special Focus on Cancer. Asian Pacific Journal of Tropical Biomedicine, 5, 182-189. https://doi.org/10.1016/S2221-1691(15)30003-4
Zamperlini, G.C.M., Silva, M.R.S. and Vilegas, W. (1997) Identification of Polycyclic Aromatic Hydrocarbons in Sugar Cane Soot by Gas Chromatography-Mass Spectrometry. Chromatographia, 46, 655-663. https://doi.org/10.1007/BF02490527
[9]
Cakir, C., Budak, G., Karabulut, A. and Sahin, Y. (2003) Analysis of Trace Elements in Different Three Region Coals in Erzurum (Turkey): A Study Using EDXRF. Journal of Quantitative Spectroscopy and Radiative Transfer, 76, 101-106. https://doi.org/10.1016/S0022-4073(02)00048-1
[10]
Park, J.H. and Penning, T.M. (2009) Polyaromatic Hydrocarbons. In: Process-Induced Food Toxicants: Occurrence, Formation, Mitigation, and Health Risks, John Wiley and Sons, Hoboken, 243-282. https://doi.org/10.1002/9780470430101.ch2h
[11]
Mackay, D., Shiu, W.Y. and Ma, K.C. (1992) Illustrated Handbook of Physical-Chemical Properties and Environmental Fate for Organic Chemicals. Vol. 2, Lewis Publishers, Chelsea.
[12]
Schirmer, K., Dixon, D.G., Greenberg, B.M. and Bols, N.C. (1998) Ability of 16 Priority PAHs to Be Directly Cytotoxic to a Cell Line from the Rainbow Trout Gill. Toxicology, 127, 129-141. https://doi.org/10.1016/S0300-483X(98)00030-4
[13]
Sun, F., Littlejohn, D. and Gibson, M.D. (1998) Ultrasonication Extraction and Solid Phase Extraction Clean-Up for Determination of US EPA 16 Priority Pollutant Polycyclic Aromatic Hydrocarbons in Soils by Reversed-Phase Liquid Chromatography with Ultraviolet Absorption Detection. Analytica Chimica Acta, 364, 1-11. https://doi.org/10.1016/S0003-2670(98)00186-X
[14]
Zamperlini, G.C., Santiago-Silva, M. and Vilegas, W. (2000) Solid-Phase Extraction of Sugar Cane Soot Extract for Analysis by Gas Chromatography with Flame Ionisation and Mass Spectrometric Detection. Journal of Chromatography A, 889, 281-286. https://doi.org/10.1016/S0021-9673(00)00291-0
[15]
Meena, R. and Paulraj, R. (2012) Oxidative Stress Mediated Cytotoxicity of TiO2 Nano Anatase in Liver and Kidney of Wistar Rat. Toxicological & Environmental Chemistry, 94, 146-163. https://doi.org/10.1080/02772248.2011.638441
[16]
Meena, R., Kumar, S., Gaharwar, U.S. and Rajamani, P. (2017) PLGA-CTAB Curcumin Nanoparticles: Fabrication, Characterization and Molecular Basis of Anticancer Activity in Triple Negative Breast Cancer Cell Lines (MDA-MB-231 Cells). Biomedicine & Pharmacotherapy, 94, 944-954. https://doi.org/10.1016/j.biopha.2017.07.151
[17]
Vacha, R., Cechmankova, J. and Skala, J. (2010) Polycyclic Aromatic Hydrocarbons in Soil and Selected Plants. Plant, Soil and Environment, 56, 434-443. https://doi.org/10.17221/7/2010-PSE
[18]
Bahurudeen, A. and Santhanam, M. (2015) Influence of Different Processing Methods on the Pozzolanic Performance of Sugarcane Bagasse Ash. Cement and Concrete Composites, 56, 32-45. https://doi.org/10.1016/j.cemconcomp.2014.11.002
[19]
Norsuraya, S., Fazlena, H. and Norhasyimi, R. (2016) Sugarcane Bagasse as a Renewable Source of Silica to Synthesize Santa Barbara Amorphous-15 (SBA-15). Procedia Engineering, 148, 839-846. https://doi.org/10.1016/j.proeng.2016.06.627
[20]
Dogan, O., Symsek, Ö., Nuhoglu, Y., Kopya, M. and Ertugrul, M. (2001) X-Ray Fluorescence Spectrometry Analysis of Trace Elements in Fly Ash Samples of KemerkÖy Thermal Power Plants. Journal of Trace and Microprobe Techniques, 19, 289-295. https://doi.org/10.1081/TMA-100002218
[21]
Malik, M., Soni, N.K., Kanagasabapathy, V., Prasad, M.V.R. and Satpathy, K.K. (2016) Characterisation of Fly Ash from Coal Fired Thermal Power Plants Using Energy Dispersive X-Ray Fluorescence Spectrometry. Scientific Reviews and Chemical Communications, 6, 91-101.
[22]
Ayanda, O.S., Fatoki, O.S., Adekola, F.A. and Ximba, B.J. (2012) Characterization of Fly Ash Generated from Matla Power Station in Mpumalanga, South Africa. Journal of Chemistry, 9, 1788-1795. https://doi.org/10.1155/2012/451801
[23]
Raychoudhury, S.S. and Kubinski, D. (2003) Polycyclic Aromatic Hydrocarbon-Induced Cytotoxicity in Cultured Rat Sertoli Cells Involves Differential Apoptotic Response. Environmental Health Perspectives, 111, 33-38. https://doi.org/10.1289/ehp.5458
[24]
Wilk, A., Waligórski, P., Lassak, A., Vashistha, H., Lirette, D., Tate, D. and Estrada, J.J. (2013) Polycyclic Aromatic Hydrocarbons—Induced ROS Accumulation Enhances Mutagenic Potential of T Antigen from Human Polyomavirus JC. Journal of Cellular Physiology, 228, 2127-2138. https://doi.org/10.1002/jcp.24375
[25]
Tarantini, A., Maitre, A., Lefebvre, E., Marques, M., Marie, C., Ravanat, J.L. and Douki, T. (2009) Relative Contribution of DNA Strand Breaks and DNA Adducts to the Genotoxicity of Benzo [a] Pyrene as a Pure Compound and in Complex Mixtures. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 671, 67-75. https://doi.org/10.1016/j.mrfmmm.2009.08.014
[26]
Woo, S., Kim, S., Yum, S., Yim, U.H. and Lee, T.K. (2006) Comet Assay for the Detection of Genotoxicity in Blood Cells of Flounder (Paralichthys olivaceus) Exposed to Sediments and Polycyclic Aromatic Hydrocarbons. Marine Pollution Bulletin, 52, 1768-1775. https://doi.org/10.1016/j.marpolbul.2006.08.027
[27]
Berge, G., Mollerup, S., Hewer, A., Phillips, D.H., Eilertsen, E. and Haugen, A. (2004) Role of Estrogen Receptor in Regulation of Polycyclic Aromatic Hydrocarbon Metabolic Activation in Lung. Lung Cancer, 45, 289-297. https://doi.org/10.1016/j.lungcan.2004.02.014
[28]
Kwack, S.J. and Lee, B.M. (2000) Correlation between DNA or Protein Adducts and Benzo[a]pyrene Diol Epoxide I-Triglyceride Adduct Detected in Vitro and in Vivo. Carcinogenesis, 21, 629-632. https://doi.org/10.1093/carcin/21.4.629
[29]
Yang, F., Zhang, Q., Guo, H. and Zhang, S. (2010) Evaluation of Cytotoxicity, Genotoxicity and Teratogenicity of Marine Sediments from Qingdao Coastal Areas Using in Vitro Fish Cell Assay, Comet Assay and Zebrafish Embryo Test. Toxicology in Vitro, 24, 2003-2011. https://doi.org/10.1016/j.tiv.2010.07.019
[30]
Matzenbacher, C.A., Garcia, A.L.H., dos Santos, M.S., Nicolau, C.C., Premoli, S., Corrêa, D.S. and Kalkreuth, W. (2017) DNA Damage Induced by Coal Dust, Fly and Bottom Ash from Coal Combustion Evaluated Using the Micronucleus Test and Comet Assay in Vitro. Journal of Hazardous Materials, 324, 781-788. https://doi.org/10.1016/j.jhazmat.2016.11.062
