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- 2018
淋巴管密度、微血管密度与VEGF在胃癌中的生物学意义
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Abstract:
摘要 目的: 探讨血管内皮生长因子(VEGF)和与淋巴管密度(LVD)和微血管密度(MVD)之间的关系以及在胃癌组织中的临床病理意义.方法: 应用免疫组化SP法检测29例正常胃组织、43例不典型增生胃组织和48例胃癌中VEGF的表达水平,同时标记D2-40和CD105,计数淋巴管密度(LVD)、微血管密度(MVD).结果:(1)在胃癌组织内的VEGF,MVD,LVD较正常胃组织、不典型增生胃组织明显增高,且均有统计学差异(P＜0.05);(2)胃癌组织中VEGF的表达在有淋巴结转移组高于无转移组;浸润深度T3、T4组也高于T1、T2组,有统计学差异(P＜0.05);MVD在有淋巴结转移组高于无转移组;浸润深度T3、T4组也高于T1、T2组,有统计学差异(P＜0.05);LVD在有淋巴结转移组高于无转移组;浸润深度T3、T4组也高于T1、T2组且高、中分化组LVD高于低分化组有统计学差异(P＜0.05);(3)胃癌组织中,VEGF的阳性表达率为77%,在VEGF阳性组中的MVD计数明显高于在阴性组中的计数,有统计学差异(P＜0.05),而在LVD则无统计学差异((P＞0.05).结论: VEGF可能通过促进胃癌的血管形成而参与癌细胞的浸润转移,VEGF和MVD、LVD的检测可作为胃癌转移的指标.
| [1] | GIORGADZE T A,BALOCH Z W,PASHA T,et al.Lymphatic and blood vessel density in the follicular patterned lesions of thyroid.d[J].Mod Pathol,2005,18(11):1424-1431. |
| [2] | 万琳琳, 赵光明, 展瑞,等. D2-40标记微淋巴管密度对乳腺癌淋巴结转移的临床意义[J]. 现代肿瘤医学, 2015,15:2136-2138. |
| [3] | WANG L L,ZHANO G M,ZHAN R, et al. Detection of D2-40 marked micro-lymphatic vessel density in the tissues of human breast cancer[J]. Journal of Modern Oncology, 2015,15:2136-2138. |
| [4] | SUNDOV Z,TOMIC S,ALFIREVIC S,et al. Prognostic value of MVD,LVDand vascular invasion in lymph node-negative colon cancer[J].Hepato-Gastroenterology,2013, 60(123):432-438. |
| [5] | WEIDNER N,SPMPLE J P,WELCH W R,et al.Tumor angiogenesis and metastasis-correlation in invasive breast carcinoma[J].N Engl J Med,1991,324(1):1-8. |
| [6] | GRUDZINSKI M,CAMBRUZZI E,LAHUDE E,et al.Cox-2 and CDl05 expression in breast cancer and disease-free survival[J].Rev Assoc Med Bras,2006,52(4):275-280. |
| [7] | DING S,LI C,LIN S,et al.Comparative evaluation of microvessel density deternmined by CD34 or CDl05 in benign and malignant gastric lesions[J].Hum Pathol,2006,37(5):861-866. |
| [8] | NIU B H,HUANGPU C S,ZHAO W et al. Relationship between CD105 and MVD of colorectal carainoma[J]. Journal of Henan University(Medical Science),2008,27(2):24-25. |
| [9] | CEBE-SUAREZ S,ZEHNDER-FJALLMAN A. The role of VEGF receptors in angiogenesis; complex partnerships[J].Cellular and Molecular Life Sciences, 2006,63(5):601-615. |
| [10] | REINDERS M E,SHO M,IZAWA A,et al.Prointlammatory functions of vaseula endothelial growth factor in alloimmunity[J].J Clin Invest,2003,112(11):1655-1665. |
| [11] | 陈秀慧, 曲军英. Ang-2及VEGF在早期宫颈癌中的表达及与血管及淋巴管密度的相关性[J]. 福建医药杂志, 2011, 33(1):4-7. |
| [12] | 刘新兰,李亦工,马志兰. 胃癌组织中VEGF-C、VGFR-3表达和微淋巴管密度的研究[J]. 宁夏医学杂志,2014,36(5):385-387;384. |
| [13] | SAHARINEN P, TAMMELA T, KARKKAINEN M J, et al. Lymphatic vasculature: development, molecular regulation and role in tumor metastasis and inflammation[J]. Trends in Immunology, 2004, 25(7):387-395. |
| [14] | KARPANEN T, ALITALO K. Lymphatic vessels as targets of tumor therapy[J]. Journal of Experimental Medicine, 2001, 194(6):F37-42. |
| [15] | 乔宏,杜进荣,付红,等 子宫内膜样癌微血管密度及血管内皮生长因子表达的研究[J].哈尔滨医科大学学报,2002,36(1):23-25. |
| [16] | QIAO H,DU J R,FU H, et al. Study of microvessel density and the expression of vascular endothelial growth factor in endometrial carcinoma[J] Journal of Harbin Medical University, 2002,36(1):23-25. |
| [17] | 王玥,刘爱东,庞久玲,等.CDl05在结直肠腺癌组织中的表达及意义[J].山东医药,2007,47(20):140-141. |
| [18] | WANG Y,LIU A D,PANG J L, et al. Expression and clinicopathological significance of CD105 in colorectal cancer[J]. Shandong Medical Journal, 2007,47(20):140-141. |
| [19] | LIU X L,LI Y G,MA Z L, et al. A study on expression of VEGF-C and VEGFR-3 and study on the lymphatic microvessel density in gastric carcinoma[J]. Ningxia Medical Journal,2014,36(5):385-387;384. |
| [20] | AGARWAL B,SAXENA R,MORIMIYA A,et al.Lymphangiogenesis does not occur in breast cancer[J].Am J Surg Pathol,2005,29(11):1449-1455. |
| [21] | YAO Y,PAN Y,CHEN J,et al.Endodin(CDl05) expression in angiogenesis of primary hepatocellular carcinomas:analysis tissue microarrays and comparisons with CD34 and VEGF[J]. Ann Clin Lab Sic,2007,37(1):39-48. |
| [22] | 牛保华,皇甫超申,赵卫,等.CD105及微血管密度与胃癌的关系[J].河南大学学报(医学版),2008,27(2):24-25. |
| [23] | SUBHOJIT R,ALBERT C,JOHN Q,et al. D2-40, a novel monoclonal antibody against the M2A antigen as a marker to distinguish hemangioblastomas from renal cell carcinomas[J].Acta Neuropathologica,2005,109(5):497-502. |
| [24] | MARKS A,SUTHERLAND D R,BAILEY D,et al.Characterization and distribution of an oncofetal antigen(M2A antigen) expressed on testicular germ cell tumours[J]. Br J Cancer,1999,80(3/4):569-578. |
| [25] | PUSZTASZERI M P,SEELENTAG W,et al.Immunohistochemical expression of endothelial markers CD31,CD34,yon Willebrand factor and Fli-1 in nonnal human tissues[J].Journal of Histochemistry and Cytochemistry,2006,54(4):385-395. |
| [26] | HOMSI J,DAUD A I.Spectrum of activity and mechanism of action of VEGF/PDGF inhibitors[J].Cancer Control,2007,14(3):285-294. |
| [27] | CHEN X H,QU J Y. Correlation between expression of Ang-2 and VEGF and the density of new blood vessels and lymphatic at early stage of cervical cancer development[J]. Fujian Medical Journal, 2011, 33(1):4-7. |
| [28] | KATO Y, ASANO K, MOGI T, et al.Clinical sig nificance of circulating vascular endothelial growth factor in dogs with mammary gland tumors[J].Vet Med Sci, 2007, 69(1):77-80. |
| [29] | TOLL A, GIMENO-BELTRN J, FERRANDIZ-PULIDO C, et al. D2-40 immunohistochemical overexpression in cutaneous squamous cell carcinomas: a marker of metastatic risk[J]. Journal of the American Academy of Dermatology, 2012, 67(6):1310-1318. |
