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- 2018
环氧树脂LX-120固定化脂肪酶
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Abstract:
摘要 目的: 探究国产环氧树脂LX-120固定化脂肪酶的工业化应用潜力. 方法: 使用环氧树脂LX-120对脂肪酶进行共价固定化研究,通过正交实验优化了固定化条件并考察了固定化酶的操作稳定性. 结果: 最佳固定化条件为:反应时间3 h,反应温度30 ℃,pH 6.5,给酶量600 U.由上述条件制备所得的固定化酶酶活为(357.03±7.09) U/g,酶活回收率高达95.42%.与游离酶相比,固定化酶的热稳定性和酸碱稳定性均有明显提升.连续反应10次,固定化酶的酶活仍保留64.21%,操作稳定性较好;4 ℃下储存30 d,固定化脂肪酶仍保留78.14%的活力. 结论: 环氧树脂LX-120在脂肪酶等工业酶的固定化技术中有较好的应用前景.
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