沈阳地区油松枝枯病病原菌的鉴定
Pathogen Identification of the Shoot Blight of Chinese Pine in Shenyang

采用传统形态分类学和现代分子生物学（DNA测序）相结合的方法，对沈阳地区近些年发生的油松枯枝病病原菌进行了分类鉴定研究，对筛选的2种真菌的子实体进行了接种试验。结果表明，在沈阳地区油松发病枯死枝上分离到的6个菌株（A1、A2、A3、A4、B1与B2）为2种菌，其中，菌种A为铁锈薄盘菌（Cenangium ferruginosum Fr. ex Fr.），菌种B为松丛赤壳菌（Nectria cucurbitula Sacc.）。2种真菌对油松健康小枝条均有侵染致病性，并分别获得有性子实体，证明了2种真菌为引起油松枯枝病的病原菌。
Traditional morphological taxonomy and modern molecular biology（DNA sequencing） were combined to classify and identify pathogenic fungi of Chinese pine shoot blight in Shenyang. Six strains（No. A1-A4，B1-B2） were isolated on dead branches of Pinus tabulaeformis, belonging to two fungus species. Strain A was Cenangium ferruginosum Fr.ex.Fr, strain B was Nectria cucurbitula Sacc. Two fungus species all presented pathogenicity in small healthy branches of P. tabulaeformis. The sexual fruiting bodies were obtained, indicating 2 species of fungi belonged to pathogenic fungi for P. tabulaeformis branches disease