高温胁迫对小麦花药叶绿素质量分数及RuBP羧化酶基因表达的影响
Effects of High-temperature Stress on Chlorophyll Mass Fraction and RuBP Carboxylase Gene Expression in Wheat (Triticum aestivum L.)

为了揭示高温胁迫下小麦花药叶绿素质量分数的变化规律及与RuBP羧化酶的关系，以正常生长条件下和高温胁迫下不同发育时期的花药（单核后期、二核期和三核期）为供试材料，比较分析小麦花药中叶绿素质量分数、RuBP羧化酶活性及其基因的表达水平。结果表明，自单核后期开始，高温胁迫下，花药中叶绿素质量分数显著低于相应的对照，并随着花药发育，这种差异幅度不断加大。而相对于叶绿素a、叶绿素b对温度则更为敏感，其变化幅度更为显著；同时在花药发育的整个时期内，处理植株的RuBP羧化酶活性及其基因的表达量也均显著低于对照植株。由此可见，高温胁迫严重破坏花药中叶绿体的光合作用系统，导致花药自养能力丧失且对外来营养物质的加工合成过程受阻，影响花药发育过程中物质供应的短缺，引起花药的败育。
To reveal the chlorophyll concentration change in wheat anthers and its relationship with RuBP carboxylase (RuBPcase),wheat anthers treated by high-temperature and control materials at different developmental stages (later-uninucleate stage,binucleate stage and trinucleate stage) were used to study the chlorophyll mass fraction,RuBPcase activity and related gene expression. The results suggested that the concentration of chlorophyll decreased under high-temperature condition,and chlorophyll b was more sensitive to high-temperature stress than chlorophyll a; In addition,the activity of RuBPcase and its gene expression level decreased significantly in treated materials. These results suggested that the photosynthetic system in anthers could be destroyed under high-temperature,and this caused loss of autotrophic ability in anthers,the synthesis and accumulation of starch grains maintaining pollen grain development were blocked,and finally resulted in the abortion of anthers.