LncRNA MEG3对骨肉瘤细胞增殖、侵袭和凋亡的影响
Effect of LncRNA MEG3 on proliferation, invasion and apoptosis in osteosarcoma cells

目的:探讨LncRNA MEG3(MEG3)对骨肉瘤细胞增殖、侵袭和凋亡的影响。方法:构建pcDNA3.0-MEG3重组质粒。qRT-PCR法检测MEG3在人正常成骨细胞hFOB1.19,转染pcDNA3.0-MEG3前后人骨肉瘤细胞系MG63、U-2 OS中的表达,以转染空质粒和未转染的骨肉瘤细胞作对照。采用CCK-8法检测转染空质粒和pcDNA3.0-MEG3的MG63、U-2 OS细胞的增殖能力,采用Transwell迁移实验和流式细胞术分别检测细胞迁移能力和细胞凋亡。结果:与正常成骨细胞相比,MEG3在骨肉瘤细胞中的表达降低(P<0.05)。转染pcDNA3.0-MEG3重组质粒后MG63和U-2 OS细胞的增殖和侵袭能力减弱,凋亡增强(P<0.05)。结论:MEG3在骨肉瘤细胞中低表达; MEG3过表达可抑制骨肉瘤细胞增殖和侵袭,促进凋亡。
Aim: To investigate the effect of LncRNA MEG3(MEG3)on the proliferation, invasion and apoptosis of osteosarcoma cells.Methods: The expression of MEG3 in normal human osteoblast hFOB1.19 and human osteosarcoma cell line MG63 and U-2 OS were detected by qRT-PCR. We constructed the recombinant plasmid of pcDNA3.0-MEG3. Then the empty plasmid and the recombinant plasmids were transfected into human osteosarcoma cell lines, the expression of MEG3 in MG63 and U-2 OS was detected by qRT-PCR, and cell proliferation, migration and apoptosis were detected by CCK-8 assay, transwell migration assay and flow cytometry assay, respectively.Results: Compared with hFOB1.19, the expressions of MEG3 in osteosarcoma cells were significantly decreased(P<0.05). The abilities of proliferation and invasion of MG63 and U-2 OS cells transfected with pcDNA3.0-MEG3 were significantly reduced, and the cell apoptosis was significantly increased(P<0.05).Conclusion: MEG3 is downregulated in osteosarcoma cells, and the overexpression of MEG3 can inhibit the proliferation and invasion, and promote apoptosis of osteosarcoma cells