高血压大鼠心肌组织中睾酮受体蛋白的表达
Expression of testosterone receptor in myocardial tissue from hypertensive rats

目的：探讨高血压心肌重构中睾酮受体表达水平改变的意义。方法：以“两肾一夹”法制备高血压大鼠模型。实验分为3组：对照组（n=10，不给予任何处理）、假手术组（n=10）和模型组（n=10）。术后8周，HE染色观察大鼠心肌组织形态学变化，免疫组化法检测心肌组织中睾酮受体及丝裂素活化蛋白激酶磷酸酶1（MKP？？1）蛋白的表达。结果：与对照组和假手术组相比，模型组心肌纤维排列紊乱，部分心肌纤维断裂，心肌表现为不同程度肥大或变性，睾酮受体及MKP？？1蛋白表达水平降低（P＜0.05），且二者存在线性正相关关系（r=0.657，P=0.039）。结论：睾酮受体参与了高血压心肌重构的过程，其作用机制可能与丝裂素活化蛋白激酶信号转导通路有关。
Aim: To explore the role of testosterone receptor in hypertension myocardial remodeling.Methods: Thirty rats were allocated into three groups:control group，sham operation group，and model group（the rats were to establish hypertension model）,with ten rats in each group.Eight weeks after operation,the morphological change in myocardial tissue was observed under light microscope, and the expression levels of testosterone receptor and MKP？？1 were tested by immunohistochemistry.Results: Compared with the sham operation group and control group, the myocardial fibers of model group disarrayed with some broken,varying degrees of myocardial hypertrophy of degeneration,and the expression levels of testosterone receptor and MKP？？1 in myocardial tissue decreased significantly（P＜0.05）,and they were positively correlated（r=0.657，P=0.039）.Conclusion: The testosterone receptor might take part in myocardial remodeling which links with the MAPKs signal transduction pathway